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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.7
- 수록면
- 473 - 481 (9page)
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초록· 키워드
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Background: Mitochondrial dysfunction is one of the significant reasons for Alzheimer"s disease (AD). Ginsenosides, natural molecules extracted from Panax ginseng, have been demonstrated to exert essential neuroprotective functions, which can ascribe to its anti-oxidative effect, enhancing central metabolism and improving mitochondrial function. However, a comprehensive analysis of cellular mitochondrial bioenergetics after ginsenoside treatment under Aβ-oxidative stress is missing.
Methods: The antioxidant activities of ginsenoside Rb₁, Rd, Re, Rg₁ were compared by measuring the cell survival and reactive oxygen species (ROS) formation. Next, the protective effects of ginsenosides of mitochondrial bioenergetics were examined by measuring oxygen consumption rate (OCR) in PC12 cells under Aβ-oxidative stress with an extracellular flux analyzer. Meanwhile, mitochondrial membrane potential (MMP) and mitochondrial dynamics were evaluated by confocal laser scanning microscopy.
Results: Ginsenoside Rg₁ possessed the strongest anti-oxidative property, and which therefore provided the best protective function to PC12 cells under the Ab oxidative stress by increasing ATP production to 3 folds, spare capacity to 2 folds, maximal respiration to 2 folds and non-mitochondrial respiration to 1.5 folds, as compared to Aβ cell model. Furthermore, ginsenoside Rg1 enhanced MMP and mitochondrial interconnectivity, and simultaneously reduced mitochondrial circularity.
Conclusion: In the present study, these results demonstrated that ginsenoside Rg₁ could be the best natural compound, as compared with other ginsenosides, by modulating the OCR of cultured PC12 cells during oxidative phosphorylation, in regulating MMP and in improving mitochondria dynamics under Aβ-induced oxidative stress.
Methods: The antioxidant activities of ginsenoside Rb₁, Rd, Re, Rg₁ were compared by measuring the cell survival and reactive oxygen species (ROS) formation. Next, the protective effects of ginsenosides of mitochondrial bioenergetics were examined by measuring oxygen consumption rate (OCR) in PC12 cells under Aβ-oxidative stress with an extracellular flux analyzer. Meanwhile, mitochondrial membrane potential (MMP) and mitochondrial dynamics were evaluated by confocal laser scanning microscopy.
Results: Ginsenoside Rg₁ possessed the strongest anti-oxidative property, and which therefore provided the best protective function to PC12 cells under the Ab oxidative stress by increasing ATP production to 3 folds, spare capacity to 2 folds, maximal respiration to 2 folds and non-mitochondrial respiration to 1.5 folds, as compared to Aβ cell model. Furthermore, ginsenoside Rg1 enhanced MMP and mitochondrial interconnectivity, and simultaneously reduced mitochondrial circularity.
Conclusion: In the present study, these results demonstrated that ginsenoside Rg₁ could be the best natural compound, as compared with other ginsenosides, by modulating the OCR of cultured PC12 cells during oxidative phosphorylation, in regulating MMP and in improving mitochondria dynamics under Aβ-induced oxidative stress.
목차
abstract
1. Introduction
2. Methods
3. Results
4. Discussion
References
참고문헌 (15)
참고문헌 신청
Eikelenboom P / 2000 / Neuroinflammation and Alzheimer disease : clinical and therapeutic implications / Alzheimer Dis Assoc Disord 14(Supplement):S54~S61
LeBlanc A / 1994 / The role of b-amyloid peptide in Alzheimer's disease / Metab Brain Dis 9(1):3~31
김종훈 / 2018 / Pharmacological and medical applications of Panax ginseng and ginsenosides: a review for use in cardiovascular diseases / Journal of Ginseng Research 42(3):264~269
Qi LW / 2011 / Ginsenosides from American ginseng : chemical and pharmacological diversity / Phytochemistry 72(8):689~699
Saw CLL / 2012 / Pharmacodynamics of ginsenosides : antioxidant activities, activation of Nrf2, and potential synergistic effects of combinations / Chem Res Toxicol 25(8):1574~1580
LeBlanc A / 1994 / The role of b-amyloid peptide in Alzheimer's disease / Metab Brain Dis 9(1):3~31
김종훈 / 2018 / Pharmacological and medical applications of Panax ginseng and ginsenosides: a review for use in cardiovascular diseases / Journal of Ginseng Research 42(3):264~269
Qi LW / 2011 / Ginsenosides from American ginseng : chemical and pharmacological diversity / Phytochemistry 72(8):689~699
Saw CLL / 2012 / Pharmacodynamics of ginsenosides : antioxidant activities, activation of Nrf2, and potential synergistic effects of combinations / Chem Res Toxicol 25(8):1574~1580
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