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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.7
- 수록면
- 204 - 204 (1page)
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The COVID-19 pandemic is caused by SARS-CoV-2, which continues to raise, public health concerns worldwide. Coronaviruses have an outer crown-like spike protein that binds to the human Angiotensin-converting enzyme 2 (ACE2) gene, resulting in infection via endocytosis. Therefore, research on hACE2 is expected to be critical for developing our understaining of COVID-19. To facilitate this, we developed Yucatan miniature pigs expressing hACE2 as animal models for COVID-19 research. First, vector containing hACE2 gene, FLAG tag, and GFP was constructed using the CMV promoter. Three lines of stable cell lines with hACE2 protein expression were created by transfecting Yucatan miniature pig ear fibroblasts with the constructed vector. The established cells then underwent somatic cell nuclear transfer, and were transferred to surrogate sows as donor cells, resulting in the succ ... 전체 초록 보기
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