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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제8호
- 발행연도
- 2020.1
- 수록면
- 1,244 - 1,251 (8page)
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Phospholipase A2 (PLA2) from Streptomyces violaceoruber is a lipolytic enzyme used in a wide range of industrial applications including production of lysolecithins and enzymatic degumming of edible oils. We have therefore investigated expression and secretion of PLA2 in two workhorse microbes, Pichia pastoris and Escherichia coli. The PLA2 was produced to an activity of 0.517 ± 0.012 U/ml in the culture broth of the recombinant P. pastoris. On the other hand, recombinant E. coli BL21 star (DE3), overexpressing the authentic PLA2 (P-PLA2), showed activity of 17.0 ± 1.3 U/ml in the intracellular fraction and 21.7 ± 0.7 U/ml in the culture broth. The extracellular PLA2 activity obtained with the recombinant E. coli system was 3.2-fold higher than the corresponding value reached in a previous study, which employed recombinant E. coli BL21 (DE3) overexpressing codon-optimized PLA2. Finally, we observed that the extracellular PLA2 from the recombinant E. coli P-PLA2 culture was able to hydrolyze 31.1 g/l of crude soybean lecithin, an industrial substrate, to a conversion yield of approximately 95%. The newly developed E. coli-based PLA2 expression system led to extracellular production of PLA2 to a productivity of 678 U/l·h, corresponding to 157-fold higher than that obtained with the P. pastoris-based system. This study will contribute to the extracellular production of a catalytically active PLA2.
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참고문헌 (33)
참고문헌 신청
Cerminati S / 2019 / Industrial uses of phospholipases : current state and future applications / Appl. Microbiol. Biotechnol 103:2571~2582
De Maria L / 2007 / Phospholipases and their industrial applications / Appl. Microbiol. Biotechnol 74:290~300
Dijkstra AJ / 2010 / Enzymatic degumming / Eur. J. Lipid Sci. Technol 112:1178~1189
Noel JP / 1989 / Phospholipase A2 engineering : design, synthesis, and expression of a gene for bovine(pro)phospholipase A2 / J. Cell Biochem 40:309~320
van den Bergh CJ / 1987 / Secretion of biologically active porcine prophospholipase A2 by Saccharomyces cerevisia : use of the prepro sequence of the alpha-mating factor / Eur. J. Biochem 170:241~246
De Maria L / 2007 / Phospholipases and their industrial applications / Appl. Microbiol. Biotechnol 74:290~300
Dijkstra AJ / 2010 / Enzymatic degumming / Eur. J. Lipid Sci. Technol 112:1178~1189
Noel JP / 1989 / Phospholipase A2 engineering : design, synthesis, and expression of a gene for bovine(pro)phospholipase A2 / J. Cell Biochem 40:309~320
van den Bergh CJ / 1987 / Secretion of biologically active porcine prophospholipase A2 by Saccharomyces cerevisia : use of the prepro sequence of the alpha-mating factor / Eur. J. Biochem 170:241~246
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