지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제9호
- 발행연도
- 2020.1
- 수록면
- 1,430 - 1,435 (6page)
이용수
초록· 키워드
오류제보하기
Bacterial cellulose (BC) has outstanding physical and chemical properties, including high crystallinity, moisture retention, and tensile strength. Currently, the major producer of BC is Komagataeibacter xylinus. However, due to limited tools of expression, this host is difficult to engineer metabolically to improve BC productivity. In this study, a regulated expression system for K. xylinus with synthetic ribosome binding site (RBS) was developed and used to engineer a BC biosynthesis pathway. A synthetic RBS library was constructed using green fluorescent protein (GFP) as a reporter, and three synthetic RBSs (R4, R15, and R6) with different strengths were successfully isolated by fluorescence-activated cell sorting (FACS). Using synthetic RBS, we optimized the expression of three homologous genes responsible for BC production, pgm, galU, and ndp, and thereby greatly increased it under both static and shaking culture conditions. The final titer of BC under static and shaking conditions was 5.28 and 3.67 g/l, respectively. Our findings demonstrate that reinforced metabolic flux towards BC through quantitative gene expression represents a practical strategy for the improvement of BC productivity.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Zhong C / 2013 / Metabolic flux analysis of Gluconacetobacter xylinus for bacterial cellulose production / Appl. Microbiol. Biotechnol 97:6189~6199
Mangayil R / 2017 / Engineering and characterization of bacterial nanocellulose films as low cost and flexible sensor material / ACS Appl. Mater. Interfaces 9:19048~19056
Ha JH / 2011 / Bacterial cellulose production from a single sugar α-linked glucuronic acid-based oligosaccharide / Process Biochem 46:1717~1723
Sajadi E / 2019 / Increased cellulose production by heterologous expression of bcsA and B genes from Gluconacetobacter xylinus in E. coli Nissle 1917 / Bioprocess Biosyst. Eng. 42:2023~2034
Buldum G / 2018 / Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli / Bioprocess Biosyst. Eng 41:265~279
Mangayil R / 2017 / Engineering and characterization of bacterial nanocellulose films as low cost and flexible sensor material / ACS Appl. Mater. Interfaces 9:19048~19056
Ha JH / 2011 / Bacterial cellulose production from a single sugar α-linked glucuronic acid-based oligosaccharide / Process Biochem 46:1717~1723
Sajadi E / 2019 / Increased cellulose production by heterologous expression of bcsA and B genes from Gluconacetobacter xylinus in E. coli Nissle 1917 / Bioprocess Biosyst. Eng. 42:2023~2034
Buldum G / 2018 / Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli / Bioprocess Biosyst. Eng 41:265~279
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