Towards a Miniaturized Culture Screening for Cellulolytic Fungi and Their Agricultural Lignocellulosic Degradation

Jantima Arnthong; Chatuphon Siamphan; Charuwan Chuaseeharonnachai; Nattawut Boonyuen; Surisa Suwannarangsee

추천

검색

자료유형: 학술저널

저자정보: Jantima Arnthong (National Center for Genetic Engineering and Biotechnology (BIOTEC)) Chatuphon Siamphan (National Center for Genetic Engineering and Biotechnology (BIOTEC)) Charuwan Chuaseeharonnachai (National Center for Genetic Engineering and Biotechnology (BIOTEC)) Nattawut Boonyuen (National Center for Genetic Engineering and Biotechnology (BIOTEC)) Surisa Suwannarangsee (National Center for Genetic Engineering and Biotechnology (BIOTEC))

저널정보: 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제11호

발행연도: 2020.1

수록면: 1,670 - 1,679 (10page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

The substantial use of fungal enzymes to degrade lignocellulosic plant biomass has widely been attributed to the extensive requirement of powerful enzyme-producing fungal strains. In this study, a two-step screening procedure for finding cellulolytic fungi, involving a miniaturized culture method with shake-flask fermentation, was proposed and demonstrated. We isolated 297 fungal strains from several cellulose-containing samples found in two different locations in Thailand. By using this screening strategy, we then selected 9 fungal strains based on their potential for cellulase production. Through sequence-based identification of these fungal isolates, 4 species in 4 genera were identified: Aspergillus terreus (3 strains: AG466, AG438 and AG499), Penicillium oxalicum (4 strains: AG452, AG496, AG498 and AG559), Talaromyces siamensis (1 strain: AG548) and Trichoderma afroharzianum (1 strain: AG500). After examining their lignocellulose degradation capacity, our data showed that P. oxalicum AG452 exhibited the highest glucose yield after saccharification of pretreated sugarcane trash, cassava pulp and coffee silverskin. In addition, Ta. siamensis AG548 produced the highest glucose yield after hydrolysis of pretreated sugarcane bagasse. Our study demonstrated that the proposed two-step screening strategy can be further applied for discovering potential cellulolytic fungi isolated from various environmental samples. Meanwhile, the fungal strains isolated in this study will prove useful in the bioconversion of agricultural lignocellulosic residues into valuable biotechnological products.

#Lignocellulosic biomass #microplate-based screening #enzymatic saccharification #cellulase #hemicellulase #fungal degradation

참고문헌 (60)

참고문헌 신청

Mohanty AK / 2002 / Sustainable Bio-Composites from renewable resources : Opportunities and challenges in the green materials world / J. Polym. Environ 10:19~26

Bessou C / 2011 / Biofuels, greenhouse gases and climate change. A review / Agron. Sustain. Dev. 31:1

Dessie W / 2020 / Current advances on waste biomass transformation into value-added products / Appl. Microbiol. Biotechnol 104:4757~4770

Tripathi N / 2019 / Biomass waste utilisation in low-carbon products : harnessing a major potential resource / NPJ Climate Atmosph. Sci 2:35

Lin CY / 2020 / Strategies for the production of biochemicals in bioenergy crops / Biotechnol. Biofuels 13:71

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!