지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Marker-assisted backcrossing is a powerful method for developing new cultivars. To develop genomic-wide markers, genotyping-by-sequencing(GBS) can be an efficient method. However, unrefined low-quality markers and missing data between markers can contribute to hamperingthe marker selection process, particularly in multi-way crosses. In this study, we aimed to calculate the recovery rate of offspring individualsand minimize errors that occur among a large number of markers. Initially, missing data were imputed by comparing samples using the k-nearestneighbor (k-NN) algorithm. Thereafter, low-quality single-nucleotide polymorphisms (SNPs) were corrected by applying the graphical representationmethod based on the k-NN algorithm in order of the SNPs in a chromosome designed for a multi-parental population. Four-way cross anddouble-backcrossed tomato BC1F1 (230 lines) and BC2F1 (96 lines) populations were genotyped by GBS. The genotype of samples of theBC1F1 and BC2F1 populations was determined based on the parental haplotype. Thus, the method of visualizing the genotype of offspringindividuals, generated via crosses of multiple parents, not only improves estimation of the recovery rate but also facilitates easier selectionin breeding programs.
목차
등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
Browning SR / 2007 / Rapid and accurate haplotype phasing and missing-data inference for wholegenome association studies by use of localized haplotype clustering / Am J Hum Genet 81:1084~1097
Collard BC / 2008 / Marker-assisted selection : an approach for precision plant breeding in the twenty-first century / Philos Trans R Soc Lond B Biol Sci 363:557~572
Cox MP / 2010 / SolexaQA : At-a-glance quality assessment of Illumina second-generation sequencing data / BMC Bioinformatics 11:485
Crossa J / 2013 / Genomic prediction in maize breeding populations with genotypingby-sequencing / G3-Genes Genom Genet 3:1903~1926
de Haas LS / 2017 / Low-coverage resequencing detects meiotic recombination pattern and features in tomato RILs / DNA Res 24:549~558
Collard BC / 2008 / Marker-assisted selection : an approach for precision plant breeding in the twenty-first century / Philos Trans R Soc Lond B Biol Sci 363:557~572
Cox MP / 2010 / SolexaQA : At-a-glance quality assessment of Illumina second-generation sequencing data / BMC Bioinformatics 11:485
Crossa J / 2013 / Genomic prediction in maize breeding populations with genotypingby-sequencing / G3-Genes Genom Genet 3:1903~1926
de Haas LS / 2017 / Low-coverage resequencing detects meiotic recombination pattern and features in tomato RILs / DNA Res 24:549~558
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