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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충북대학교 동물의학연구소 Journal of Biomedical and Translational Research Journal of Biomedical and Translational Research 제21권 제4호
- 발행연도
- 2020.1
- 수록면
- 152 - 164 (13page)
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초록· 키워드
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Quantitative real-time polymerase chain reaction (RT- qPCR) is a rapid and precise method of analysis to quantify the level of gene expression and is widely used in the diagnosis of diseases and quantitative analysis of genes. In RT-qPCR analysis, a reference gene (or housekeeping gene) is used for normalization of experimental results. Since this method of analysis detects a small quantity of the product, it is highly sensitive and it is important for the accuracy and reproducibility of the experiment to select a reference gene suitable for gene expression studies. As the expression levels of the reference gene are affected under different conditions, in order to determine the suitability of the housekeeping gene used as the reference gene, it is necessary to verify the expression stability. In the current study, the stability of the expression of 11 housekeeping genes (B2M, SDHA, GAPDH, RPL13, VIM, EEF1A1, HPRT1, GUSB, RPL19, ACTB, and ABL1) was investigated in the tissues of long-tailed chickens (heart, thigh, and breast). Expression stability evaluation was analyzed with four software: BestKeeper, NormFinder, geNorm, and RefFinder. In our study, GAPDH in heart tissue, HPRT1 in thigh tissue, and RPL13 in breast tissue were selected as the most stable reference genes. Evaluation of the expression stability of housekeeping genes can provide important data in gene expression studies by selecting an appropriate reference gene according to various conditions.
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참고문헌 (30)
참고문헌 신청
Zhang J / 2018 / Selection of housekeeping genes for quantitative gene ex-pression analysis in yellow-feathered broilers / Ital J Anim Sci 17:540~546
Shi C / 2016 / Evaluation of housekeeping genes for quantitative real-time PCR analysis of Bradysia odoriphaga(Diptera : Sciarida) / Int J Mol Sci 17:1034
남윤권 / 2018 / 러시아 철갑상어(Acipenser gueldenstaedtii) 발생 시료의 RT-qPCR 분석을 위한 내재 대조군 유전자의 선정 / 한국수산과학회지 51(1):95~106
Lü J / 2018 / Selection of reference genes for the normalization of RT-Qpcr data in gene expression studies in inseclts : a systematic review / Front Physiol 9:1560
Selvarajah GT / 2017 / Reference gene validation for gene expression normalization in canine osteosarcoma : a geNorm algorithm approach / BMC Vet Res 13:354
Shi C / 2016 / Evaluation of housekeeping genes for quantitative real-time PCR analysis of Bradysia odoriphaga(Diptera : Sciarida) / Int J Mol Sci 17:1034
남윤권 / 2018 / 러시아 철갑상어(Acipenser gueldenstaedtii) 발생 시료의 RT-qPCR 분석을 위한 내재 대조군 유전자의 선정 / 한국수산과학회지 51(1):95~106
Lü J / 2018 / Selection of reference genes for the normalization of RT-Qpcr data in gene expression studies in inseclts : a systematic review / Front Physiol 9:1560
Selvarajah GT / 2017 / Reference gene validation for gene expression normalization in canine osteosarcoma : a geNorm algorithm approach / BMC Vet Res 13:354
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