Comparative transcriptomic and physiological analyses of contrasting hybrid cultivars ND476 and ZX978 identify important differentially expressed genes and pathways regulating drought stress tolerance in maize

Guo Liu; Tinashe Zenda; Songtao Liu; Xuan Wang; Hongyu Jin; Anyi Dong; Yatong Yang; Huijun Duan

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자료유형: 학술저널

저자정보: Guo Liu (Hebei Agricultural University) Tinashe Zenda (Hebei Agricultural University) Songtao Liu (Hebei Agricultural University) Xuan Wang (Hebei Agricultural University) Hongyu Jin (Hebei Agricultural University) Anyi Dong (Hebei Agricultural University) Yatong Yang (Hebei Agricultural University) Huijun Duan (Hebei Agricultural University)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.42 No.8

발행연도: 2020.1

수록면: 937 - 955 (19page)

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연구주제

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연구배경

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연구방법

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Background Drought is the major abiotic stress factor that negatively influences growth and yield in cereal grain crops such as maize (Zea mays L.). A multitude of genes and pathways tightly modulate plant growth, development and responses to environmental stresses including drought. Therefore, crop breeding efforts for enhanced drought resistance require improved knowledge of plant drought responses. Objective Here, we sought to elucidate the molecular and physiological mechanisms underpinning maize drought stress tolerance. Methods We therefore applied a 12-day water-deficit stress treatment to maize plants of two contrasting (drought tolerant ND476 and drought sensitive ZX978) hybrid cultivars at the late vegetative (V12) growth stage and performed a large-scale RNA sequencing (RNA-seq) transcriptome analysis of the leaf tissues. Results A comparative analysis of the two genotypes leaf transcriptomes and physiological parameters revealed the key differentially expressed genes (DEGs) and metabolic pathways that respond to drought in a genotype-specific manner. A total of 3114 DEGs were identified, with 21 DEGs being specifically expressed in tolerant genotype ND476 in response to drought stress. Of these, genes involved in secondary metabolites biosynthesis, transcription factor regulation, detoxification and stress defense were highly expressed in ND476. Physiological analysis results substantiated our RNA-seq data, with ND476 exhibiting better cell water retention, higher soluble protein content and guaiacol peroxidase activity, along with low lipid peroxidation extent than the sensitive cultivar ZX978 under drought conditions. Conclusion Our findings enrich the maize genetic resources and enhance our further understanding of the molecular mechanisms regulating drought stress tolerance in maize. Additionally, the DEGs screened in this study may provide a foundational basis for our future targeted cloning studies.

#Differentially expressed genes (DEGs) · Drought stress · Secondary metabolites biosynthesis · RNA sequencing (RNA-seq) · Transcriptome profiles · Zea mays L

참고문헌 (80)

참고문헌 신청

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