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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 1,023 - 1,033 (11page)
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초록· 키워드
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Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.
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참고문헌 (27)
참고문헌 신청
Cheong C / 2006 / Role of INK4a locus in normal eye development and cataract genesis / Mech Ageing Dev 127:633~638
Dalke C / 2004 / Electroretinography as a screening method for mutations causing retinal dysfunction in mice / Investig Ophthalmol Vis Sci 45:601~609
Doetschman T / 2009 / Influence of genetic background on genetically engineered mouse phenotypes / Methods Mol Biol(Clifton, NJ) 530:423~433
Gaj T / 2013 / ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering / Trends Biotechnol 31:397~405
Hendrich HJ / 2012 / The laboratory mouse / Elsevier
Dalke C / 2004 / Electroretinography as a screening method for mutations causing retinal dysfunction in mice / Investig Ophthalmol Vis Sci 45:601~609
Doetschman T / 2009 / Influence of genetic background on genetically engineered mouse phenotypes / Methods Mol Biol(Clifton, NJ) 530:423~433
Gaj T / 2013 / ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering / Trends Biotechnol 31:397~405
Hendrich HJ / 2012 / The laboratory mouse / Elsevier
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