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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 1,291 - 1,298 (8page)
이용수
초록· 키워드
오류제보하기
Background Pseudobagrus brevicorpus is an endangered species in Korea. The development of genetic markers and geneticinformation regarding the populations of this species are needed to establish conservation strategies. Objective As part of the conservation of P. brevicorpus, a 12-microsatellite marker was developed using next-generationsequencing (NGS) to provide current genetic population information. Methods Microsatellites from P. brevicorpus were identified by NGS analysis. Genetic diversity and genetic structureanalysis of six populations (Seojeong Stream [SJ], Gokgang Stream [GK], Jaho Stream [JH], Daega Stream [DG], NamRiver [NG], and Deokcheon River [DC]) of P. brevicorpus were conducted using the newly developed microsatellite marker. Results NGS generated 10,347,578 reads and identified 659,507 simple sequence repeats. Twelve microsatellites were successfullyamplified and verified in 30 individuals of P. brevicorpus. The genetic diversity of the six P. brevicorpus populationsin terms of the number of alleles ranged from 3.667 to 7.111. All populations except DG deviated significantly fromHardy–Weinberg equilibrium (HWE) at one or more loci. The genetic distances of the six populations showed the closestrelationship between the SJ and GK populations (independent Stream populations), and there was a close relationship withthe JH population among the Nakdong River. Structure analysis showed that P. brevicorpus is largely divided into two groups. Conclusions The developed microsatellite marker will be used to provide basic genetic data of P. brevicorpus. Genetic diversityand structure analysis of the population will provide useful information for conservation management of P. brevicorpus
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참고문헌 (45)
참고문헌 신청
Benchimol LL / 2007 / Structure of genetic diversity among common bean (Phaseolus vulgaris L.)varieties of Mesoamerican and Andean origins using new developed microsatellite markers / Genet Resour Crop Evol 54:1747~1762
Botstein D / 1980 / Construction of a genetic linkage map in man using restriction fragment length polymorphisms / Am J Hum Genet 32:314~331
변화근 / 2009 / 한국산 둑중개속 어류 개체군들의 형태 변이 및 AFLP 분석을 통한 유전 변이 / 한국어류학회지 21(2):67~75
Castoe TA / 2010 / Rapid identification of thousands of copperhead snake(Agkistrodon contortrix)microsatellite loci from modest amounts of 454 shotgun genome sequence / Mol Ecol Resour 10:341~347
Csencsics D / 2010 / Cost-effective, species-specific microsatellite development for the endangered dwarf bulrush(Typha minima)using next generation sequencing technology / J Hered 101:789~793
Botstein D / 1980 / Construction of a genetic linkage map in man using restriction fragment length polymorphisms / Am J Hum Genet 32:314~331
변화근 / 2009 / 한국산 둑중개속 어류 개체군들의 형태 변이 및 AFLP 분석을 통한 유전 변이 / 한국어류학회지 21(2):67~75
Castoe TA / 2010 / Rapid identification of thousands of copperhead snake(Agkistrodon contortrix)microsatellite loci from modest amounts of 454 shotgun genome sequence / Mol Ecol Resour 10:341~347
Csencsics D / 2010 / Cost-effective, species-specific microsatellite development for the endangered dwarf bulrush(Typha minima)using next generation sequencing technology / J Hered 101:789~793
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