지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 243 - 250 (8page)
이용수
초록· 키워드
오류제보하기
We have developed a novel paper-based molecular pathogen diagnostic method by combining the direct loop-mediated isothermal amplification (Direct LAMP) in a paper reactor and an immunochromatographic strip (ICS) detection without any DNA preparation step. The mineral paper is durable, oil- and tear-resistant, and waterproof, so it is a proper material for point-of-care (POC) testing owing to facile chip fabrication, cost-effectiveness, and disposability. The mineral paper was utilized as a substrate to construct the reactor for gene amplification based on the principle of origami without using an expensive and complicated process. In this novel paper reactor, we performed the Direct LAMP reaction, which is a direct isothermal DNA amplification without any sample preparation step such as cell lysis, DNA extraction and purification for amplifying specific target gene. After the Direct LAMP reaction in a paper reactor, a capillary tube was employed to take an appropriate amount (~ 2 mL) of amplicons in order to transfer the LAMP product to an ICS. The use of the ICS allowed us to perform the simple and rapid colorimetric detection of the resultant amplified target genes by naked eyes, thereby confirming the presence of the pathogen in a sample in a user-friendly way. In this platform, we could simultaneously identify Staphylococcus aureus and Escherichia coli O157:H7 contaminated in human blood or milk. The whole molecular diagnostic process (the Direct LAMP in a mineral paper plus the ICS detection) could be conducted in a paper substrate, so the proposed method would be ideal for pathogen detection in resourcelimited environments.
목차
등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Scallan, E. / 2011 / Foodborne illness acquired in the United States-Major pathogens / Emerging Infect. Dis 17:7~15
Jung, J. H. / 2012 / Ultrafast Rotary PCR system for multiple influenza viral RNA detection / Lab Chip 12:1598~1600
Niemz, A. / 2011 / Point-ofcare nucleic acid testing for infectious diseases / Trends Biotechnol 29:240~250
Wiedbrauk, D. L. / 1995 / Inhibition of PCR by aqueous and vitreous fluids / J. Clin. Microbiol 33:2643~2646
Oh, S. J. / 2016 / Fully automated and colorimetric foodborne pathogen detection on an integrated centrifugal microfluidic device / Lab Chip 16:1917~1926
Jung, J. H. / 2012 / Ultrafast Rotary PCR system for multiple influenza viral RNA detection / Lab Chip 12:1598~1600
Niemz, A. / 2011 / Point-ofcare nucleic acid testing for infectious diseases / Trends Biotechnol 29:240~250
Wiedbrauk, D. L. / 1995 / Inhibition of PCR by aqueous and vitreous fluids / J. Clin. Microbiol 33:2643~2646
Oh, S. J. / 2016 / Fully automated and colorimetric foodborne pathogen detection on an integrated centrifugal microfluidic device / Lab Chip 16:1917~1926
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