개인구독
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지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 275 - 283 (9page)
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초록· 키워드
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BACKGROUND: Vitamin is a well-known co-factor for many metabolic processes and its roles in fertility and follicular growth have been studied. Vitamin supplementation is frequently achieved by daily ingestion in the form of a complex capsule. However, the role of single and complex vitamins in in vitro maturation of murine follicles is not fully elucidated. METHODS: In this study, we evaluated the effects of two forms of vitamins. Pure L-ascorbic acid, and multi-vitamin (vitamin C ? vitamin B complex) was treated at two different concentrations (50 and 100 lg/ml), to pre-puberty murine follicles during in vitro maturation. To determine the specific stage of growth that is affected by treatment with vitamins, the vitamins were treated from day 0, 4, 9, and 13. Growth of each follicle was assessed by measuring diameters of whole expanded area and of the granulosa cells. Expression of follicular and oocyte growth-related genes and the effect of vitamin on the viability of follicles was assessed using senescence associated b-galactosidase staining. RESULTS: Treatment with vitamins promoted the in vitro growth of murine follicles and the upregulated the expression of granulosa cell- and oocyte-specific genes such as BMP15, Fsh receptor, and GDF9. The proliferation of the granulosa cells was enhanced by the treatment of vitamin. Fifty lg/ml concentration vitamin showed greater effects compared to higher concentration. The viability of in vitro grown follicles was also significantly improved in vitamin-treated follicles. The effects of single L-ascorbic acid and complex vitamin were not significantly different to those of day 4 and day 9 follicles. Vitamins promoted murine follicle development in vitro with different effects on specific growth stage. CONCLUSION: Supplementation of vitamins during in vitro maturation of murine follicles is an efficient strategy for in vitro expansion of follicular cells. These results could be customized to the sophisticated culture of follicles retrieved from aged or cancer-survived female that contain smaller number of follicles with reduced potential to develop into mature follicles.
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참고문헌 (36)
참고문헌 신청
Markstro¨m E / 2002 / Survival factors regulating ovarian apoptosis—dependence on follicle differentiation / Reproduction 123:23~30
Kim YJ / 2013 / MicroRNAs transfected into granulosa cells may regulate oocyte meiotic competence during in vitro maturation of mouse follicles / Hum Reprod 28:3050~3061
Espey LL / 1976 / The distribution of collagenous connective tissue in rat ovarian follicles / Biol Reprod 14:502~506
Park KE / 2013 / Effects of urinary and recombinant gonadotropins on in vitro maturation outcomes of mouse preantral follicles / Reprod Sci 20:909~916
Kim YJ / 2017 / Induction of multiple ovulation via modulation of angiotensin II receptors in in vitro ovarian follicle culture models / J Tissue Eng Regen Med 11:3100~3110
Kim YJ / 2013 / MicroRNAs transfected into granulosa cells may regulate oocyte meiotic competence during in vitro maturation of mouse follicles / Hum Reprod 28:3050~3061
Espey LL / 1976 / The distribution of collagenous connective tissue in rat ovarian follicles / Biol Reprod 14:502~506
Park KE / 2013 / Effects of urinary and recombinant gonadotropins on in vitro maturation outcomes of mouse preantral follicles / Reprod Sci 20:909~916
Kim YJ / 2017 / Induction of multiple ovulation via modulation of angiotensin II receptors in in vitro ovarian follicle culture models / J Tissue Eng Regen Med 11:3100~3110
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