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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 315 - 323 (9page)
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초록· 키워드
오류제보하기
Single nucleotide polymorphisms (SNPs) are abundantly and evenly distributed throughout the genomes of most plantspecies. These markers have become popular for use in genetic research in many crops. SNP markers can be used to screen maizecultivars rapidly during the early growth stages. In this study, to develop additional SNP markers for maize, we chose 20 SNP sites perchromosome from the maizeGDB website (www.maizegdb.org) and designed primers with two base pair mismatches using PrimerDesigner 4 based on putative SNP sites of the B73 genome sequence. The polymerase chain reaction (PCR) products ranged from 200to 500 bp in size, whereas no PCR product was detected when the SNP site was present in Korean waxy corn. Using nine Koreancommercial F1 hybrids of waxy corn, including Chalok 1, Chalok 4, Ilmichal, Eolrukchal 1, Heukjinjuchal, Hayanchal 95, Mibaekchal,Mibaek 2, and Miheukchal, we selected 16 primer sets showing clear bands or no bands. Based on cluster analysis, we confirmed thatthe nine Korean waxy corn hybrids could clearly be distinguished. The SNP marker sets are easy to utilize through simple PCR andagarose gel electrophoresis. These results suggest that analysis using the SNP marker set designed in this study would be faster, cheaper,and more reproducible than that using other genotyping tools, such as cleaved amplified polymorphic sequence markers, which requirethe use of restriction enzymes.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Ahmadian A / 2000 / Single-nucleotide polymorphism analysis by pyrosequencing / Anal. Biochem 280:103~110
Alderborn A / 2000 / Determination of single-nucleotide polymorphisms by real-time pyrophosphate DNA sequencing / Genome Res 10:1249~1258
Azanza F / 1996 / Quantitative trait loci influencing chemical and sensory characteristics of eating quality in sweet corn / Genome 39:40~50
Carey EE / 1984 / Sugar characteristics of sweet corn populations from a sugary enhancer breeding program / Euphytica 33:609~622
Choe ES / 2012 / Genetic and QTL analysis of pericarp thickness and ear architecture traits of Korean waxy corn germplasm / Euphytica 183:243~260
Alderborn A / 2000 / Determination of single-nucleotide polymorphisms by real-time pyrophosphate DNA sequencing / Genome Res 10:1249~1258
Azanza F / 1996 / Quantitative trait loci influencing chemical and sensory characteristics of eating quality in sweet corn / Genome 39:40~50
Carey EE / 1984 / Sugar characteristics of sweet corn populations from a sugary enhancer breeding program / Euphytica 33:609~622
Choe ES / 2012 / Genetic and QTL analysis of pericarp thickness and ear architecture traits of Korean waxy corn germplasm / Euphytica 183:243~260
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