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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 757 - 766 (10page)
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Polyploid breeding is an effective method that has been used widely for the development of new varieties of plants. MicroRNAs (miRNAs) are a class of endogenous small RNAs that regulate gene expression at the posttranscriptional level. To understand the functions of miRNAs in autotetraploid and diploid Paulownia ‘Yuza 1’ plants, two small RNA libraries and two degradome sequencing libraries of the autotetraploid and the corresponding diploid plants were constructed and analyzed. A total of 49 conserved miRNAs that were grouped into 15 families, and 25 novel miRNAs were obtained from two sRNA libraries. Among these miRNAs, 28 were differentially expressed at a significant level in the autotetraploid relative to the diploid. Moreover, the miRNA target genes were identified by degradome sequencing and some of the differently expressed miRNAs and their target genes were validated by quantitative RT-PCR analysis. This study has provided valuable information on P. ‘Yuza 1’ miRNAs and their targets, which will enrich the Paulownia miRNA information resources and will contribute to our understanding of miRNA-mediated regulation in autotetraploid Paulownia plantlets.
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참고문헌 (37)
참고문헌 신청
Addo-Quaye C / 2008 / Endogenous siRNA and miRNA targets identified by sequencing of the Arabidopsis degradome / Curr Biol 18:758~762
Audic S / 1997 / The significance of digital gene expression profiles / Genome Res 7:986~995
Chen C / 2005 / Real-time quantification of microRNAs by stem-loop RT-PCR / Nucleic Acids Res 33:e179
Ding D / 2009 / Differential expression of miRNAs in response to salt stress in maize roots / Ann Bot 103:29~38
Doebley J / 1997 / The evolution of apical dominance in maize / Nature 386:485~488
Audic S / 1997 / The significance of digital gene expression profiles / Genome Res 7:986~995
Chen C / 2005 / Real-time quantification of microRNAs by stem-loop RT-PCR / Nucleic Acids Res 33:e179
Ding D / 2009 / Differential expression of miRNAs in response to salt stress in maize roots / Ann Bot 103:29~38
Doebley J / 1997 / The evolution of apical dominance in maize / Nature 386:485~488
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