Transcriptome analysis of male and female mature gonads of Japanese scallop Patinopecten yessonsis

Jun Ding; Dan Yang; Chao Yin; Yaqing Chang; Yan Dou; Zhenlin Hao

추천

검색

자료유형: 학술저널

저자정보: Jun Ding (Dalian Ocean University) Dan Yang (Dalian Ocean University) Chao Yin (Dalian Ocean University) Yaqing Chang (Dalian Ocean University) Yan Dou (Dalian Ocean University) Zhenlin Hao (Dalian Ocean University)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.38 No.11

발행연도: 2016.1

수록면: 1,041 - 1,052 (12page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

The Japanese scallop Patinopecten yessoensis is an important commercial culture shellfish in China and Japan. In addition to its commercial interest, it has also attracted much attention because of its value in studying sex determination and differentiation mechanisms. Herein, two transcriptome libraries from male and female gonads at maturing stage were constructed. The two libraries were paired-end sequenced using Illumina sequencing techniques. A total of 81,986,898 cleaned reads were obtained, and assembled into 29,897 unigenes by using Trinity software and removing redundancy. Compared with the public Swiss-Prot and NR databases by BlastX, 9354 unigenes were significantly matched to known unique proteins. As determined by GO annotation and KEGG pathway mapping, functional annotation of the unigenes uncovered diverse biological functions and processes. After comparing the two transcriptome libraries, 8652 upregulated and 2973 down-regulated genes were identified in the male gonads. According to annotation information, at least 30 genes related to sex determination and differentiation, such as Dmrt1, Sox9, fem1 and vasa, were identified and characterized. The expression patterns of the random eight genes were then validated by quantitative real-time PCR (qRT-PCR) suggesting the high reliability of RNA-Seq results. The study provides an archive for further studies of molecular mechanisms of bivalve sex determination and differentiation.

참고문헌 (69)

참고문헌 신청

Audic S / 1997 / The significance of digital gene expression profiles / Genome Res 7:986~995

Bairoch A / 1991 / The SWISS-PROT protein sequence data bank / Nucleic Acids Res 19:2247

Benjamini Y / 2001 / The control of the false discovery rate in multiple testing under dependency / Ann Stat 29:1165~1188

Benjamini Y / 2005 / Quantitative trait loci analysis using the false discovery rate / Genetics 171:783~790

Chang CC / 2002 / Akam M(2002)Germ line development in the grasshopper Schistocerca gregaria : vasa as a marker / Dev Biol 252:100~118

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!