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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 155 - 160 (6page)
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초록· 키워드
오류제보하기
A small and air-breathing snail, Ellobium chinense (Ellobiidae), is a vulnerable species by International Union for Conservation of Nature (IUCN). To protect and manage habitat and population of E. chinense, microsatellite markers were developed using 454 pyrosequencing and 20 polymorphic microsatellite markers were identified. A total of 146,704 sequences containing a minimum of four repeat motifs (mean, 631 base pairs) were identified from 499,505 reads. Among 80 loci containing more than nine repeat units, 34 primer sets (42.5 %) produced strong PCR products, of which 20 were polymorphic among 48 samples of E. chinense. All loci exhibited high genetic variability, with an average of 18.9 alleles per locus, and the mean observed and expected heterozygosities were 0.65 and 0.90, respectively. In addition, cross-amplification was tested for all 20 loci in the same family species, Melampus sincaporensis. None of the primer pairs resulted in effective amplification, which might be due to their high mutation rates. Our work demonstrated the utility of next-generation 454 sequencing as a method for the rapid and cost-effective identification of microsatellites. The high degree of polymorphism exhibited by the 20 newly developed microsatellites will be useful in future conservation genetic studies of this species.
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참고문헌 (28)
참고문헌 신청
An HS / 2012 / Development of microsatellite markers for the Korean mussel, Mytilus coruscus (Mytilidae) using next-generation sequencing / Int J Mol Sci 13:10583~10593
An HS / 2012 / Application of novel polymorphic microsatellite loci identified in the Korean Pacific Abalone [Haliotis diversicolor supertexta (Haliotidae)] in the genetic characterization of wild and released populations / Int J Mol Sci 13:10750~10764
An CM / 2013 / New polymorphic microsatellite loci of threadsail filefish, Stephanolepis cirrhifer (Teleostei, Monacanthidae), from Korean waters / Genet Mol Res 12:1679~1690
Association of Wildlife Research and Envision / Search system of Japanese Red Data
Berman M / 2014 / Characterization of the complete mitochondrial genome and 13 microsatellite loci through next-generation sequencing for the New Caledonian spider-ant Leptomyrmex pallens / Mol Biol Rep 41:1179~1187
An HS / 2012 / Application of novel polymorphic microsatellite loci identified in the Korean Pacific Abalone [Haliotis diversicolor supertexta (Haliotidae)] in the genetic characterization of wild and released populations / Int J Mol Sci 13:10750~10764
An CM / 2013 / New polymorphic microsatellite loci of threadsail filefish, Stephanolepis cirrhifer (Teleostei, Monacanthidae), from Korean waters / Genet Mol Res 12:1679~1690
Association of Wildlife Research and Envision / Search system of Japanese Red Data
Berman M / 2014 / Characterization of the complete mitochondrial genome and 13 microsatellite loci through next-generation sequencing for the New Caledonian spider-ant Leptomyrmex pallens / Mol Biol Rep 41:1179~1187
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