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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 957 - 965 (9page)
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초록· 키워드
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Glu-D1y12.K as a novel y-type subunit was found in HMW-GSs encoded at the Glu-D1 locus in the JB20, which a Korean wheat line from F9 lines crossed by Keumkang with Glu-D1d and Chinese Spring (CS) with Glu-D1a alleles. This novel subunit shows faster electrophoretic mobility and lower molecular weight than Dy12 subunit on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The result of linear ion-trap and Fourier-transform mass spectrometry (LTQ-FT-MS) based on two-dimensional electrophoresis (2-DE) showed that the Dy12.K subunit has high similarity against protein ID: P08488 (GLT3_WHEAT) as ‘Glutenin, high molecular weight subunit 12’ form UniProtKB. The gene of the Glu-1Dy12.K subunit is composed of 1962 nucleotide base pairs containing open reading frame (ORF) as 652 amino acids corresponding to about 70.1 kDa. It has four indels (36 bp insertions: two repeated 18 and 24 bp deletion: two deletions with 6 + 18 bp) and 21 SNPs compared to Glu- 1Dy10 (GI: 164457872 in NCBI), and one deletion (18 bp) and three SNPs compared to Glu-1Dy12 (GI: 1036031968) by DNA markers. Consequentially, in comparison with Dy10, 13 SNPs were non-synonymous SNPs and eight SNPs were synonymous SNPs of 21 SNPs. In comparison with Dy12, only one SNP was non-synonymous SNP of three SNPs. Furthermore, the deduced peptide sequences as ‘TGQGQQ’ corresponding to ‘AAC AGG ACA AGG GCA ACA ’ are deleted only in the Dy12.K subunit.
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등록된 정보가 없습니다.
참고문헌 (31)
참고문헌 신청
Anderson OD / 1989 / Nucleotide sequences of the two highmolecular-weight glutenin genes from d-genome of hexaploid bread wheat, Triticum aestivum L. cv Cheyenne / Nucleic Acids Res 17:461~462
Cantu D / 2010 / Small RNAs, DNA methylation and transposable elements in wheat / BMC Genomics 11:408
Chen PD / 1994 / Transfer of Ph I genes promoting homoeologous pairing from Triticum speltoides to common wheat / Theor Appl Genet 88:97~101
D’Ovidio R / 1996 / Identification and molecular characterization of a large insertion within the repetitive domain of a high-molecular-weight glutenin subunit gene from hexaploid wheat / Theor Appl Genet 93:1048~1053
Mary Gehring / 2008 / DNA Methylation and Demethylation in Arabidopsis / The Arabidopsis Book 6:e0102
Cantu D / 2010 / Small RNAs, DNA methylation and transposable elements in wheat / BMC Genomics 11:408
Chen PD / 1994 / Transfer of Ph I genes promoting homoeologous pairing from Triticum speltoides to common wheat / Theor Appl Genet 88:97~101
D’Ovidio R / 1996 / Identification and molecular characterization of a large insertion within the repetitive domain of a high-molecular-weight glutenin subunit gene from hexaploid wheat / Theor Appl Genet 93:1048~1053
Mary Gehring / 2008 / DNA Methylation and Demethylation in Arabidopsis / The Arabidopsis Book 6:e0102
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