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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 1,297 - 1,305 (9page)
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Napier grass (Pennisetum purpureum Schum.) is an economically important species used as a fodder crop, reflecting its high biomass, perennial nature and pest resistance. In order to understand the genomic information and development of this species based on SSR markers, the transcriptome for Napier grass was obtained using nextgeneration sequencing. A total of 117,076 sequence contigs were obtained, with lengths of 201–10,652 bp. Among these contigs, 44,313 (37.85%) sequences were annotated against public protein databases (E-value <10−5). In addition, the contigs generated from the transcript sequencing were also analysed for the presence of simple sequence repeats (SSRs). A total of 12,744 SSR motifs were identified with in these contigs and corresponding primer pairs were designed. Empirical validation of a cohort of 200 SSRs was performed, with 34% being polymorphic for 20 Napier grass accessions. The polymorphic index content values for each primer ranged from 0.105 to 0.749, with an average of 0.409. The development of genetic, genomic resources and EST-SSRs for Napier grass will contribute to novel gene discovery and the marker-assisted selective breeding of this species.
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등록된 정보가 없습니다.
참고문헌 (7)
참고문헌 신청
Chen D / 2010 / Development and characterization of 47 genic microsatellite markers of the loach, Misgurnus anguillicaudatus / J World Aquac Soc 41(1):163~167
Coleman TP / 1998 / RNA secondary structure: an important cis-element in rat calcitonin/CGRP pre-messenger RNA splicing / Biochem 37(45):15941~15950
Harris K / 2010 / Genetic relationships among napiergrass (Pennisetum purpureum Schum.) nursery accessions using AFLP markers / Plant Gen Res 8(1):63~70
Kawube G / 2014 / Resistance of Napier grass clones to Napier grass stunt disease / Afr Crop Sci J 22(3):229~236
Muldoon DK / 1979 / The hybrid between Pennisetum americanum and Pennisetum purpureum / Herbage Abstracts 49(5):189~199
Coleman TP / 1998 / RNA secondary structure: an important cis-element in rat calcitonin/CGRP pre-messenger RNA splicing / Biochem 37(45):15941~15950
Harris K / 2010 / Genetic relationships among napiergrass (Pennisetum purpureum Schum.) nursery accessions using AFLP markers / Plant Gen Res 8(1):63~70
Kawube G / 2014 / Resistance of Napier grass clones to Napier grass stunt disease / Afr Crop Sci J 22(3):229~236
Muldoon DK / 1979 / The hybrid between Pennisetum americanum and Pennisetum purpureum / Herbage Abstracts 49(5):189~199
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