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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 대한노인병학회 Annals of geriatric medicine and research Annals of geriatric medicine and research Vol.21 No.2
- 발행연도
- 2017.1
- 수록면
- 78 - 85 (8page)
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Background: The aim of this study was to investigate the role of homocysteine on the proliferation of rat aortic vascular smooth muscle cells(VSMCs) by measuring mitogen-activated protein (MAP) kinase under hyperglycemic conditions. Methods: Rat aortic VSMCs were isolated from Otsuka Long-Evans Tokushima Fatty and Long-Evans Tokushima Otsuka rats. VSMCs were incubated in the presence of homocysteine(1 mM) with/without PD98059(30µM) and wortmannin (300 nM) for 48 hours in different concentrations of glucose(5.5, 25 mM). Proliferation was evaluated by methylthiazoletetrazolium(MTT), fluorescence-activated cell sorting(FACS), and western blot analyses. Results: In MTT and FACS analyses, the proliferation of VSMCs was increased by homocysteine. After PD98059(30 µM) and wortmannin(300 nM) treatment with homocysteine(1 mM), the increased proliferation of VSMC caused by homocysteine, decreased to control levels. In Western blot analysis, immunoexpression of phospho-p44/42 MAP kinase was significantly increased by homocysteine(1mM). After PD98059 and wortmannin treatment, the increased immunoexpression of phospho-p44/42 MAP kinase was suppressed. Conclusion: These results suggest that the MAP kinase and PI3 kinase pathways are the main mechanisms involved in rat VSMC proliferation caused by homocysteine under hyperglycemic conditions.
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참고문헌 (26)
참고문헌 신청
Mujumdar VS / 2002 / Mechanism of constrictive vascular remodeling by homocysteine: role of PPAR / Am J Physiol Cell Physiol 282:C1009~C1015
Jiang C / 2009 / Homocysteine promotes vascular smooth muscle cell migration by induction of the adipokine resistin / Am J Physiol Cell Physiol 297:C1466~C1476
Tan H / 2014 / Hyperhomocysteinemia promotes vascular remodeling in vein graph in mice / Front Biosci (Landmark Ed) 19:958~966
Wang G / 2000 / Homocysteine stimulates nuclear factor kappaB activity and monocyte chemoattractant protein-1 expression in vascular smooth-muscle cells: a possible role for protein kinase C / Biochem J 352(2):817~826
Refsum H / 1998 / Homocysteine and cardiovascular disease / Annu Rev Med 49:31~62
Jiang C / 2009 / Homocysteine promotes vascular smooth muscle cell migration by induction of the adipokine resistin / Am J Physiol Cell Physiol 297:C1466~C1476
Tan H / 2014 / Hyperhomocysteinemia promotes vascular remodeling in vein graph in mice / Front Biosci (Landmark Ed) 19:958~966
Wang G / 2000 / Homocysteine stimulates nuclear factor kappaB activity and monocyte chemoattractant protein-1 expression in vascular smooth-muscle cells: a possible role for protein kinase C / Biochem J 352(2):817~826
Refsum H / 1998 / Homocysteine and cardiovascular disease / Annu Rev Med 49:31~62
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