지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 262 - 271 (10page)
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초록· 키워드
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Rolling circle amplification (RCA) developed in the mid-1990s has been widely used as an efficient isothermal DNA amplification process for molecular diagnosis. This enzymatic process amplifies target DNA sequences with high fidelity and specificity by using the strand displacing DNA polymerases. The product of RCA is long single-stranded DNA that contains tandem repeat of target sequence. Isothermal reaction amplification condition of RCA has an advantage over conventional polymerase chain reaction, because no temperature cycling devices are needed for RCA. Thus, RCA is suitable tool for point-of-care detection of target nucleic acids as well as facile detection of target genes. Combined with various detection methods, RCA could amplify and detect femtomolar scale of target nucleic acids with a specificity of one or two base discrimination. Herein, RCA technology is reviewed with an emphasis on molecular diagnosis of microRNAs, infectious pathogens, and point mutations.
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참고문헌 (49)
참고문헌 신청
Fire, A. / 1995 / Rolling replication of short DNA circles / Proc. Natl. Acad. Sci. USA 92:4641~4645
Liu, D. / 1996 / Rolling Circle DNA Synthesis: Small Circular Oligonucleotides as Efficient Templates for DNA Polymerases / J. Am. Chem. Soc 118:1587~1594
Blanco, L. / 1984 / Characterization and purification of a phage phi 29-encoded DNA polymerase required for the initiation of replication / Proc. Natl. Acad. Sci. USA 81:5325~5329
Blanco, L. / 1989 / Highly efficient DNA synthesis by the phage phi 29 DNA polymerase. Symmetrical mode of DNA replication / J. Biol. Chem 264:8935~8940
Beyer, S. / 2005 / Periodic DNA nanotemplates synthesized by rolling circle amplification / Nano Lett 5:719~722
Liu, D. / 1996 / Rolling Circle DNA Synthesis: Small Circular Oligonucleotides as Efficient Templates for DNA Polymerases / J. Am. Chem. Soc 118:1587~1594
Blanco, L. / 1984 / Characterization and purification of a phage phi 29-encoded DNA polymerase required for the initiation of replication / Proc. Natl. Acad. Sci. USA 81:5325~5329
Blanco, L. / 1989 / Highly efficient DNA synthesis by the phage phi 29 DNA polymerase. Symmetrical mode of DNA replication / J. Biol. Chem 264:8935~8940
Beyer, S. / 2005 / Periodic DNA nanotemplates synthesized by rolling circle amplification / Nano Lett 5:719~722
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