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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 83 - 92 (10page)
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Simultaneous visualization of numerous proteins in cells and tissues is fundamental for understanding their diversity and dynamics and to address the heterogeneity of human diseases. Despite the many advantages of multiplex analysis, current methods typically rely upon immunohistochemistry, which can only be used to detect a few proteins. The exceptional optical properties of nanoparticles (NPs) have improved multiplex imaging in cells and tissues. Here, we review recent advances in multiplex imaging-based molecular diagnosis using antibody-conjugated NPs (immuno-NPs). With the aim of furthering development, we anticipate that NP-based multiplex imaging techniques will complement classical immunohistochemistry methods and provide insight into the biological diversity of cells and tissues.
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참고문헌 (57)
참고문헌 신청
Crivat, G. / 2012 / Imaging proteins inside cells with fluorescent tags / Trends Biotechnol. 30:8~16
Beck, M. / 2011 / The quantitative proteome of a human cell line / Mol. Syst. Biol. 7:549
Wu, L. / 2013 / Variation and genetic control of protein abundance in humans / Nature 499:79~82
Stack, E.C. / 2014 / Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis / Methods 70:46~58
Elshal, M.F. / 2006 / Multiplex bead array assays: performance evaluation and comparison of sensitivity to ELISA / Methods 38:317~323
Beck, M. / 2011 / The quantitative proteome of a human cell line / Mol. Syst. Biol. 7:549
Wu, L. / 2013 / Variation and genetic control of protein abundance in humans / Nature 499:79~82
Stack, E.C. / 2014 / Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis / Methods 70:46~58
Elshal, M.F. / 2006 / Multiplex bead array assays: performance evaluation and comparison of sensitivity to ELISA / Methods 38:317~323
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