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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 184 - 192 (9page)
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초록· 키워드
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Here, we present a simple pipetting-based approach for generating multi-layered core-shell hydrogel droplets composed of alginate for outer shell and collagen for culturing cells inside. By using a multi-hole plastic substrate and by pipetting, multi-layered hydrogel droplets were generated in a simple and rapid manner. HEK293 cells, which are human embryonic kidney cells, were cultured for 14 days in double-layered hydrogel droplet with high viability. Cancer cells were co-cultured with epithelial cells in multi-layered hydrogel droplets and applied for drug tests with curcumin. As epithelial cells protect cancer cells from anti-cancer drugs, co-cultured cells showed lower sensitivity to curcumin. We developed a simple and easy method for creating complex hydrogel particles for 3D multicellular co-culture and developed an alternative method for drug testing in vivo.
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참고문헌 (58)
참고문헌 신청
Griffith, L.G. / 2006 / Capturing complex 3D tissue physiology in vitro / Nat. Rev. Mol. Cell Biol. 7:211~224
Pampaloni, F. / 2007 / The third dimension bridges the gap between cell culture and live tissue / Nat. Rev. Mol. Cell Biol. 8:839~845
Breslin, S. / 2013 / Three-dimensional cell culture: the missing link in drug discovery / Drug Discov. Today 18:240~249
Bhadriraju, K. / 2002 / Engineering cellular microenvironments to improve cell-based drug testing / Drug Discov. Today 7:612~620
Elliott, N.T. / 2011 / A review of three-dimensional in vitro tissue models for drug discovery and transport studies / J. Pharmaceutical Sci. 100:59~74
Pampaloni, F. / 2007 / The third dimension bridges the gap between cell culture and live tissue / Nat. Rev. Mol. Cell Biol. 8:839~845
Breslin, S. / 2013 / Three-dimensional cell culture: the missing link in drug discovery / Drug Discov. Today 18:240~249
Bhadriraju, K. / 2002 / Engineering cellular microenvironments to improve cell-based drug testing / Drug Discov. Today 7:612~620
Elliott, N.T. / 2011 / A review of three-dimensional in vitro tissue models for drug discovery and transport studies / J. Pharmaceutical Sci. 100:59~74
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