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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 287 - 293 (7page)
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Bacillus cereus can cause blood infections (i.e., sepsis). Its early detection is very important for treating patients. However, an antibody with high binding affinity to B. cereus is not currently available. Bacteriophage cell wall-binding domain (CBD) has strong and specific binding affinity to B. cereus. Here, we report the improvement in the sensitivity of an ATP bioluminescence assay for B. cereus detection using CBD-conjugated magnetic nanoparticles (CBDMNPs). The assay was able to detect as few as 10 colony forming units (CFU) per mL and 103 CFU per mL in buffer and blood. CBD-MNPs did not show any cross-reactivity with other microorganisms. These results demonstrate the feasibility of the ATP assay for the detection of B. cereus.
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참고문헌 (28)
참고문헌 신청
Sutherland, A / 2011 / Development and validation of a novel molecular biomarker diagnostic test for the early detection of sepsis / Crit. Care 15:R149
Ikeda, M / 2015 / Clinical characteristics and antimicrobial susceptibility of Bacillus cereus blood stream infections / Ann. Clin. Microbiol. Antimicrob 14:43
Goto, M / 2013 / Overall burden of bloodstream infection and nosocomial bloodstream infection in North America and Europe / Clin. Microbiol. Infect 19:501~509
Angus, D.C / 2001 / Epidemiology of severe sepsis in the United States: analysis of incidence, outcome, and associated costs of care / Crit. Care Med 29:1303~1310
Shen, H / 2016 / Rapid and selective detection of pathogenic bacteria in bloodstream infections with aptamer-based recognition / ACS Appl. Mater. Interfaces 8:19371~19378
Ikeda, M / 2015 / Clinical characteristics and antimicrobial susceptibility of Bacillus cereus blood stream infections / Ann. Clin. Microbiol. Antimicrob 14:43
Goto, M / 2013 / Overall burden of bloodstream infection and nosocomial bloodstream infection in North America and Europe / Clin. Microbiol. Infect 19:501~509
Angus, D.C / 2001 / Epidemiology of severe sepsis in the United States: analysis of incidence, outcome, and associated costs of care / Crit. Care Med 29:1303~1310
Shen, H / 2016 / Rapid and selective detection of pathogenic bacteria in bloodstream infections with aptamer-based recognition / ACS Appl. Mater. Interfaces 8:19371~19378
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