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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 554 - 559 (6page)
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The direct lineage conversion of fibroblasts into neuronal or neural precursor cells (NPCs) has become a hot issue in recent years as an attractive approach in the field of stem cell regenerative medicine. In this study, we adopted the stromal feeder co-culture method during the early conversion period to enhance conversion efficiency. Stromal cells are often used in directed differentiation of dopaminergic (DA) neurons from pluripotent stem cells. We co-cultured rat embryonic fibroblasts (REFs) on γ-irradiated sonic hedgehog-overexpressing MS5 stromal (MS5-SHH) cells after transduction with Brn2, Ascl1, Myt1L, and BclxL-GFP (BAMXGFP) transcription factors to REFs. One week after co-culture, transduced cells (GFP+ cells) that proliferated on MS5-SHH cells were separated from MS5-SHH cells through a 40 μm cell strainer. Subsequently, the converted cells (GFP+ cells) were expanded on fibronectin-coated culture plates in NPC expansion medium. The induced NPCs (iNPCs) expressed NPC potential (NESTIN+/SOX2+) earlier than seen with non-co-culture methods and were efficiently differentiated into DA neurons by overexpression of Nurr1 and Foxa2 genes, which are specific transcription factors for midbrain DA neuron development. These observations indicated that direct conversion to NPCs using an MS5 stromal cells co-culture method is a suitable technique for efficient generation of iNPC/DA neurons from fibroblasts.
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참고문헌 (25)
참고문헌 신청
Murry CE / 2008 / Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development / Cell 132:661~680
Tabar V / 2014 / Pluripotent stem cells in regenerative medicine: challenges and recent progress / Nat Rev Genet 15:82~92
Xu J / 2015 / Direct lineage reprogramming: strategies, mechanisms, and applications / Cell Stem Cell 16:119~134
Graf T / 2009 / Forcing cells to change lineages / Nature 462:587~594
Lujan E / 2012 / Direct conversion of mouse fibroblasts to self-renewing, tripotent neural precursor cells / Proc Natl Acad Sci U S A 109:2527~2532
Tabar V / 2014 / Pluripotent stem cells in regenerative medicine: challenges and recent progress / Nat Rev Genet 15:82~92
Xu J / 2015 / Direct lineage reprogramming: strategies, mechanisms, and applications / Cell Stem Cell 16:119~134
Graf T / 2009 / Forcing cells to change lineages / Nature 462:587~594
Lujan E / 2012 / Direct conversion of mouse fibroblasts to self-renewing, tripotent neural precursor cells / Proc Natl Acad Sci U S A 109:2527~2532
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