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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 195 - 209 (15page)
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Urine-derived stem cells (USCs) are considered as a promising cell source capable of neuronal differentiation. In addition, specific growth factors and extracellular matrix are essential for enhancing their neuronal differentiation effi- ciency. In this study, we investigated the possibility of neuronal differentiation of USCs and the role of laminin and platelet-derived growth factor BB (PDGF-BB) as promoting factors. USCs were isolated from fresh urine of healthy donors. Cultured USCs were adherent to the plate and their morphology was similar to the cobblestone. In addition, they showed chromosome stability, rapid proliferation rate, colony forming capacity, and mesenchymal stem cell character- istics. For inducing the neuronal differentiation, USCs were cultured for 14 days in neuronal differentiation media sup- plemented with/without laminin and/or PDGF-BB. To identify the expression of neuronal markers, RT-PCR, flow cytometry analysis and immunocytochemistry were used. After neuronal induction, the cells showed neuron-like mor- phological change and high expression level of neuronal markers. In addition, laminin and PDGF-BB respectively pro- moted the neuronal differentiation of USCs and the combination of laminin and PDGF-BB showed a synergistic effect for the neuronal differentiation of USCs. In conclusion, USCs are noteworthy cell source in the field of neuronal regeneration and laminin and PDGF-BB promote their neuronal differentiation efficiency.
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참고문헌 (49)
참고문헌 신청
Deleyrolle LP / 2009 / Isolation, expansion, and differentiation of adult Mammalian neural stem and progenitor cells using the neurosphere assay / Methods Mol Biol 549:91~101
Terenghi G / 2009 / Chapter 21: use of stem cells for improving nerve regeneration / Int Rev Neurobiol 87:393~403
Bible E / 2009 / The support of neural stem cells transplanted into stroke-induced brain cavities by PLGA particles / Biomaterials 30:2985~2994
Ahmadi N / 2012 / Stability of neural differentiation in human adipose derived stem cells by two induction protocols / Tissue Cell 44:87~94
Abdullah RH / 2016 / Induction of mice adult bone marrow mesenchymal stem cells into functional motor neuron-like cells / J Chem Neuroanat 77:129~142
Terenghi G / 2009 / Chapter 21: use of stem cells for improving nerve regeneration / Int Rev Neurobiol 87:393~403
Bible E / 2009 / The support of neural stem cells transplanted into stroke-induced brain cavities by PLGA particles / Biomaterials 30:2985~2994
Ahmadi N / 2012 / Stability of neural differentiation in human adipose derived stem cells by two induction protocols / Tissue Cell 44:87~94
Abdullah RH / 2016 / Induction of mice adult bone marrow mesenchymal stem cells into functional motor neuron-like cells / J Chem Neuroanat 77:129~142
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