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- 2021.11
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2021
Simultaneous determination of 37 lipids metabolites and Bio-profiling in human serum by ultra-high performance liquid chromatography-tandem mass spectrometry
이지선
,
윤혜란
한국분석과학회 학술대회
2021.11
학술대회자료
Simultaneous determination of 37 lipids metabolites and Bio-profiling in human serum by ultra-high performance liquid chromatography-tandem mass spectrometry
이지선
,
윤혜란
한국분석과학회 학술대회
2021.11
학술대회자료
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Biometabolites such as bile acids, omega fatty acids, sphingolipids and phospholipids are reported to be related to various inflammatory reactions such as asthma, chronic obstructive pulmonary disease(COPD), rheumatoid arthritis, and inflammatory bowel disease. We developed the ultra high performance liquid chromatography-electrospray ionization tandem mass spectrometry(UHPLC-ESI-MS/MS) based analytical method of 37 lipids metabolites(6 bile acids, 5 omega fatty acids, 2 sphingolipids, and 24 phospholipids). This method was applied to the asthma patients (n=25) and healthy controls(n=25). Statistical analyzes were performed using partial least squares discriminant analysis(PLS-DA), t-test, fold change test to effectively discriminate whether there was a difference in the concentrations of metabolites in normal and asthma groups. The result of PLS-DA showed that 16 metabolites have statistical significance with p-value <0.05. Ele^_@span style=color:#999999 ^_# ... ^_@/span^_#^_@a href=javascript:; onclick=onClickReadNode('NODE10819358');fn_statistics('Z354','null','null'); style='color:#999999;font-size:14px;text-decoration:underline;' ^_#전체 초록 보기^_@/a^_#
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