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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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초록· 키워드
오류제보하기
An alkalophilic microorganism, strain DK1122 producing an alkaline protease was identified. DK1122 was isolated from soil collected in central Korea. The strain DK1122 was Gram-positive, 0.7×2-4 μm in size, and its colony was yellowish white, The strain DK1122 was found to be spore-forming, catalase positive, oxidase positive, caseinolytic, and reduce nitrate to nitrite. The protease was produced aerobically on Horikoshi I agar medium (pH 9.0) with 1% (w/v) skim milk at 40°C for 24 h. Through 16S rRNA gene partial sequencing, the strain DK1122 had the 99.7% sequence similarity to 16S rRNA gene sequence of Bacillus pseudofirmus. Based on the biochemical and physiological properties as well as phylogenetic analysis, the isolated strain was named as Bacillus sp. DK1122. It is expected that Bacillus sp. DK1122 may be a promising candidate for a proudcer of an alkaline protease applicable to the food and detergent industries.
목차
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참고문헌 (24)
참고문헌 신청
Ahn J. / 1990 / Partial purification and characterization of alkaline protease from Bacillus sp / Korean J. Appl. Microbiol. Biotechnol 18:344~351
방성호 / 2011 / 호알칼리성 Bacillus pseudofirmus HS-54가 생산하는 알칼리성 Protease의 특성 / 미생물학회지 47(3):194~199
Cha MH. / 2001 / Isolation and characterization of the strain producing angiotensin converting enzyme inhibitor from soy sauce / J. Korean Soc. Food Sci. Nutr 30:594~599
Choi MC. / 1996 / Isolation and identification of on alkalophilic Coryneform bacterium TU-19 producing extracellular protease(s) / Korean J. Microbiol. Biotechnol 24:160~165
Delepelaire P. / 1989 / Protease secretion by Erwinia chrysanthemi / Proteases B and C are synthesized and secreted as zymogens without a signal peptide. J. Biol. Chem 264:9083~9089
방성호 / 2011 / 호알칼리성 Bacillus pseudofirmus HS-54가 생산하는 알칼리성 Protease의 특성 / 미생물학회지 47(3):194~199
Cha MH. / 2001 / Isolation and characterization of the strain producing angiotensin converting enzyme inhibitor from soy sauce / J. Korean Soc. Food Sci. Nutr 30:594~599
Choi MC. / 1996 / Isolation and identification of on alkalophilic Coryneform bacterium TU-19 producing extracellular protease(s) / Korean J. Microbiol. Biotechnol 24:160~165
Delepelaire P. / 1989 / Protease secretion by Erwinia chrysanthemi / Proteases B and C are synthesized and secreted as zymogens without a signal peptide. J. Biol. Chem 264:9083~9089
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