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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.10
- 수록면
- 169 - 176 (8page)
- DOI
- 10.15188/kjopp.2021.10.35.5.169
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이 논문의 연구 히스토리 (3)
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초록· 키워드
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This study was conducted to suggest the Cudrania tricuspidata leaf and Achyranthes japonica Nakai Complex (CAC) possibility of use as a functional natural material for improving bone disease. Cudrania tricuspidata leaf and Achyranthes japonica Nakai were mixed in the same amount, extracted with hot water, and then powdered and used in the study. After, the cytotoxicity of CAC for osteoblasts (MG63 cell), osteoclasts (differentiated RAW264.7 cell), and macrophages (RAW264.7 cell) were evaluated by MTT assay, and ALP assay and TRAP assay were performed to confirm the differentiation capacity of osteoblasts and osteoclasts, respectively. In addition, the anti-inflammatory effect in macrophages was evaluated by ELISA, qRT-PCR, and western blot assay. CAC did not proliferated osteoblasts and osteoclasts, but increased ALP activity against osteoblasts differentiation and decreased TRAP activity against osteoclasts differentiation. CAC did not proliferated macrophages but decreased nitric oxide production. Also, decreased NOS2, IL1B, IL6, PTGS2, and TNFA gene expression, and JNK and p38 protein phosphorylation in a concentration-dependent manner, but ERK protein phosphorylation was not changed. As a result, CAC increased the differentiation and activation of osteoblasts, inhibited the differentiation and activation of osteoclasts, and regulated the expression of inflammatory cytokines in macrophages. Therefore, it is thought that CAC can be used as a functional natural material that prevents bone disease and has an anti-inflammatory effect.
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재료 및 방법
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References
참고문헌 (44)
참고문헌 신청
McNally EA / 2012 / A model for the ultrastructure of bone based on electron microscopy of ion-milled sections / PloS one 7(1):e29258
Clarke B. / 2008 / Normal bone anatomy and physiology / Clinical journal of the American Society of Nephrology 3(Supplement 3):S131~S139
Maruotti N / 2017 / Osteoblast role in osteoarthritis pathogenesis / Journal of cellular physiology 232(11):2957~2963
Zhang Y / 2010 / Epidemiology of osteoarthritis / Clinics in geriatric medicine 26(3):355~369
Kanis JA / 2002 / Diagnosis of osteoporosis and assessment of fracture risk / The Lancet 359(9321):1929~1936
Clarke B. / 2008 / Normal bone anatomy and physiology / Clinical journal of the American Society of Nephrology 3(Supplement 3):S131~S139
Maruotti N / 2017 / Osteoblast role in osteoarthritis pathogenesis / Journal of cellular physiology 232(11):2957~2963
Zhang Y / 2010 / Epidemiology of osteoarthritis / Clinics in geriatric medicine 26(3):355~369
Kanis JA / 2002 / Diagnosis of osteoporosis and assessment of fracture risk / The Lancet 359(9321):1929~1936
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UCI(KEPA) : I410-ECN-0101-2022-519-000110732