메뉴 건너뛰기
.. 내서재 .. 알림
소속 기관/학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
로그인 회원가입 고객센터 ENG
주제분류

추천
검색
질문

논문 기본 정보

자료유형
학술저널
저자정보
Heejin Ham (Anyang University)
저널정보
대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY JOURNAL OF BACTERIOLOGY AND VIROLOGY Vol.52 No.3
발행연도
2022.9
수록면
120 - 127 (8page)

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
🏆
연구결과
AI에게 요청하기
추천
검색
질문

초록· 키워드

오류제보하기
Swine enteritis in all ages is caused by porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGE), rotavirus, Eimeria spp. etc., and is often fatal among neonatal piglets. This study aimed to compare the pathogenicity and nucleotide sequence of ORF3 between wild-type porcine epidemic diarrhea virus (wt-PEDV) and cell culture-adapted PEDV (ca-PEDV). A total of 30 colostrum-deprived piglets that were 1 day old were inoculated with either wt-PEDV or ca-PEDV to compare the villus height and crypt depth (VH/CD) ratio, amount of viral nucleic acid in jejunum, and open reading frame (ORF) 3 nucleotide sequence via immunofluorescence labeling of viral antigen and western blot analysis. Mean jejunal villus height and crypt depth (VH/CD) ratio in piglets infected orally with Korean strain of PEDV were 6.8 ± 0.8, 7.0 ± 0.8, 8.2 ± 1.0, 8.0 ± 1.3, and 7.9 ± 1.1 in uninfected control piglets; 7.2 ± 1.2, 5.2 ± 1.4, 2.7 ± 0.3, 1.4 ± 0.2, and 1.5 ± 0.1 in wt-PEDV-infected piglets; and 7.6 ± 1.8, 3.2 ± 0.4, 2.2 ± 0.3, 1.3 ± 0.3, and 1.1 ± 0.2 in ca-PEDV-infected piglets at 12, 24, 36, 48, and 60 h post inoculation. Conclusively, the mean VH/CD ratio for wt-PEDV piglets was significantly different from that of ca-PEDV piglets at 24, 36, and 60 h post inoculation. The in situ hybridization (ISH) positive results in piglets orally infected with the Korean strain of PEDV in jejunal villus were 4.0 ± 0.8, 10.3 ± 0.5, 7.3 ± 0.5, 7.0 ± 0.8, and 5.3 ± 0.5 in ca-PEDV-infected piglets; and 4.3 ± 0.9, 16.7 ± 1.2, 9.3 ± 0.5, 9.0 ± 0.8, and 5.7 ± 0.9 in wt-PEDV-infected piglets. Conclusively, a significant difference was observed in each group. A greater amount of PEDV nucleic acid was detected in the jejunal tissues (P < 0.05) of the ca-PEDV-inoculated piglets than in those of the wt-PEDV-infected piglets at 24, 36, and 60 h post-inoculation. The nucleotide sequences of wt-PEDV and ca-PEDV were nearly identical (98.7% homology); nucleotide substitutions were noted in ORF3 that caused some amino acid changes. Statistically significant differences were observed in the pathogenicity of ca-PEDV compared with its parental wt-PEDV; ORF3 nucleotide changes were identified in ca-PEDV that possibly influenced PEDV pathogenicity.

목차

INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

참고문헌 (0)

참고문헌 신청

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

최근 본 자료

전체보기

댓글(0)

0

UCI(KEPA) : I410-ECN-0101-2023-475-000053855