지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Melioidosis is an infection caused by Burkholderia pseudomallei and it represents one of the most common infectious diseases in zoo and farm animals giving rise to high mortality, leading to biological diversity loss. We use the bacteriological and biochemical methods such as culture, biochemical test, Gram-staining and oxidase test to examine the presence of B. pseudomallei in 50 soils treated and non-treated with quicklime powder collected from Bukit Merah Orang Utan Island, (BMOUI), Perak, Malaysia. We observed mixed bacterial colony growth with various colony morphologies. Six of the isolates labelled as P2(25), P5(2), P5(3), P5(5), P6(1), and P6(6)2 showed pinkish purple colour with wrinkled characteristics similar to B. pseudomallei colony morphology after incubation for 48 h. The results showed that all putative soil-isolated organisms were Gram-negative bacteria, positive for oxidase test and negative for catalase test. In the VITEK<SUP>®</SUP> ID system and PCR sequencing analysis, no B. pseudomallei was identified. All six putative organisms were identified as in B. cepacia complex i.e.: B. stagnalis (four cases), B. contaminans (one) and B. multivorans (one). These preliminary findings demonstrate that a few species of B. cepacia group were successfully isolated from the soil of BMOUI using selective media for B. pseudomallei.
목차
Materials and Methods
Results
Discussion
References
참고문헌 (33)
참고문헌 신청
Berriatua E, Ziluaga I, Miguel-Virto C, Uribarren P, Juste R, Laevens S, Vandamme P, and Govan JR. 2001. Outbreak of subclinical mastitis in a flock of dairy sheep associated with Burkholderia cepacia complex infection. J. Clin. Microbiol. 39, 990–994.
Brent AJ, Matthews PC, Dance DA, Pitt TL, and Handy R. 2007.
Misdiagnosing melioidosis. Emerg. Infect. Dis. 13, 349–351.
Chantratita N, Rholl DA, Sim B, Wuthiekanun V, Limmathurotsakul D, Amornchai P, Thanwisai A, Chua HH, Ooi WF, Holden MTG, et al. 2011. Antimicrobial resistance to ceftazidime involving loss of penicillin-binding protein 3 in Burkholderia pseudomallei. Proc. Natl. Acad. Sci. USA 108, 17165–17170.
Chung JW, Altman, E, Beveridge TJ, and Speert DP. 2003. Colonial morphology of Burkholderia cepacia complex genomovar III: implications in exopolysaccharide production, pilus expression, and persistence in the mouse. Infect. Immun. 71, 904–909.
Brent AJ, Matthews PC, Dance DA, Pitt TL, and Handy R. 2007.
Misdiagnosing melioidosis. Emerg. Infect. Dis. 13, 349–351.
Chantratita N, Rholl DA, Sim B, Wuthiekanun V, Limmathurotsakul D, Amornchai P, Thanwisai A, Chua HH, Ooi WF, Holden MTG, et al. 2011. Antimicrobial resistance to ceftazidime involving loss of penicillin-binding protein 3 in Burkholderia pseudomallei. Proc. Natl. Acad. Sci. USA 108, 17165–17170.
Chung JW, Altman, E, Beveridge TJ, and Speert DP. 2003. Colonial morphology of Burkholderia cepacia complex genomovar III: implications in exopolysaccharide production, pilus expression, and persistence in the mouse. Infect. Immun. 71, 904–909.
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UCI(KEPA) : I410-ECN-0101-2023-475-000155050