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자료유형
학술저널
저자정보
김근섭 (Department of Integrated Life Science and Technology Kongju National University Yesan-gun Chungnam) Park Chae-Rin (Department of Companion and Laboratory Animal Science Kongju National University Yesan-gun Chungnam) Kim Ji-Eun (Department of Companion and Laboratory Animal Science Kongju National University Yesan-gun Chungnam) Kim Hong-Kook (Department of Integrated Life Science and Technology Kongju National University Yesan-gun Chungnam) Kim Byeong-Soo (Department of Companion and Laboratory Animal Science Kongju National University Yesan-gun Chungnam)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제2호
발행연도
2022.2
수록면
220 - 227 (8page)
DOI
10.4014/jmb.2107.07053

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The spread of antibiotic-resistant strains of Staphylococcus aureus, a gram-positive opportunistic pathogen, has increased due to the frequent use of antibiotics. Inhibition of the quorum-sensing systems of biofilm-producing strains using plant extracts represents an efficient approach for controlling infections. Torilis japonica is a medicinal herb showing various bioactivities; however, no studies have reported the anti-biofilm effects of T. japonica extracts against drug-resistant S. aureus. In this study, we evaluated the inhibitory effects of T. japonica ethanol extract (TJE) on biofilm production in methicillin-sensitive S. aureus (MSSA) KCTC 1927, methicillin-resistant S. aureus (MRSA) KCCM 40510, and MRSA KCCM 40511. Biofilm assays showed that TJE could inhibit biofilm formation in all strains. Furthermore, the hemolysis of sheep blood was found to be reduced when the strains were treated with TJE. The mRNA expression of agrA, sarA, icaA, hla, and RNAIII was evaluated using reverse transcription-polymerase chain reaction to determine the effect of TJE on the regulation of genes encoding quorum sensing-related virulence factors in MSSA and MRSA. The expression of hla reduced in a concentration-dependent manner upon treatment with TJE. Moreover, the expression levels of other genes were significantly reduced compared to those in the control group. In conclusion, TJE can suppress biofilm formation and virulence factor-related gene expression in MSSA and MRSA strains. The extract may therefore be used to develop treatments for infections caused by antibiotic-resistant S. aureus.

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