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논문 기본 정보

자료유형
학술저널
저자정보
Koh Youngho (Food Microbiology Division National Institute of Food and Drug Safety Evaluation Ministry of Food a) Bae Yunyoung (Food Microbiology Division National Institute of Food and Drug Safety Evaluation Ministry of Food a) Lee Yu-Si (Food Microbiology Division National Institute of Food and Drug Safety Evaluation Ministry of Food a) Kang Dong-Hyun (Department of Food and Animal Biotechnology Department of Agricultural Biotechnology Center for Foo) Kim Soon Han (Food Microbiology Division National Institute of Food and Drug Safety Evaluation Ministry of Food a)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제10호
발행연도
2022.10
수록면
1,307 - 1,314 (8page)
DOI
10.4014/jmb.2207.07031

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In this study, we sought to investigate the various characteristics of Salmonella spp. isolated from raw chicken meats available in Korean markets. The data collected, such as food source of isolation, sampling information, serotype, virulence, and genetic profile including sequence type, were registered in the database for further comparative analysis of the strains isolated from the traceback investigation samples. To characterize serotype, virulence and gene sequences, we examined 113 domestically distributed chicken meat samples for contamination with Salmonella spp. Phylogenetic analysis was conducted on 24 strains (21.2%) of Salmonella isolated from 113 commercially available chicken meats and by-products, using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Serotyping of the isolated Salmonella spp. revealed S. Enteritidis in 11 strains (45.8%), S. Virchow in 6 strains (25%), S. Montevideo in 2 strains (8.3%), S. Bsilla in 2 strains (8.3%), S. Bareilly in 1 strain (4.2%), S. Dessau in 1 strain (4.2%), and S. Albany in 1 strain (4.2%). The genetic correlation indicated that 24 isolated strains were classified into 18 clusters with a genetic similarity of 64.4-100% between them. Eleven isolated S. Enteritidis strains were classified into 9 genotypes with a sequence identity of 74.4%, whereas the most distantly related S. Virchow was divided into five genotypes with 85.9% identity. Here, the MLST analysis indicated that the major Sequence Type (ST) of the Salmonella spp. isolated from domestic chicken sold in Chungcheong Province belongs to the ST 11 and 16, which differs from the genotype of Salmonella isolated from imported chicken. The differential sequence characteristics can be a genetic marker for identifying causative bacteria for epidemiological investigations of food poisoning.

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