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논문 기본 정보

자료유형
학술저널
저자정보
Dae-Geun Kim (Gyeongsangnam-do Fisheries Resources Research Institute) 김정현 (국립수산과학원) 백혜자 (부경대학교) 김신권 (국립수산과학원 양식관리과) Min Kwan-Sik (Hankyong National University) 김대중 (국립수산과학원)
저널정보
한국수산과학회 Fisheries and Aquatic Sciences Fisheries and Aquatic Sciences 제25권 제1호
발행연도
2022.1
수록면
12 - 19 (8page)
DOI
10.47853/FAS.2022.e2

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In the present study, we investigated the effects of recombinant eel gonadotropins (rec-GTHs) on maturation induction in immature ovarian culture in vitro and sex steroid hormones in vivo in the Japanese eel Anguilla japonica. To study the in vitro effects of rec-GTHs on estradiol-17β (E2) production in immature ovarian tissues, ovarian tissues were incubated with different doses of rec-follicle-stimulating hormone (rec-FSH) or rec-luteinizing hormone (rec-LH). The results revealed that the E2 levels in the rec-FSH (0.1, 0.5, or 1 μg/mL)- and rec-LH (0.1 or 0.5 μg/mL)-treated groups were significantly higher than those in the female eels from the control group. Furthermore, to investigate the in vivo effects of rec-GTHs on the gonadosomatic index (GSI) and plasma sex steroid hormone levels, the eels were injected intraperitoneally with eel’s ringer (control), salmon pituitary extract (SPE; for female eels), human chorionic gonadotropin (hCG; for male eels), rec-FSH, rec-LH, and rec-FSH + rec-LH once a week. The results revealed that except for the SPE and the hCG groups, none of the groups exhibited a significant difference in GSI values. However, in vivo plasma E2 levels increased at the end of 4 weeks after rec-FSH treatment in female eels. Based on these results, it is suggested that rec-GTHs may have a positive effect on sexual maturation in female eels; however, further studies on complementary rec-protein production systems and additional glycosylation of rec-hormones are needed to elucidate hormone bioactivity in vivo and in vitro.

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