지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2022.12
- 수록면
- 1,138 - 1,143 (6page)
- DOI
- 10.3349/ymj.2022.0271
이용수
초록· 키워드
오류제보하기
Purpose: Fusobacterium species can cause infections, and associations with cancer are being increasingly reported. As their clinical significance differs, accurate identification of individual species is important. However, matrix-assisted laser desorption/ion-ization-time of flight mass spectrometry has not been found to be effective in identifying Fusobacterium species in previous studies. In this study, we aimed to improve the accuracy and efficacy of identifying Fusobacterium species in clinical laboratories.
Materials and Methods: In total, 229 Fusobacterium isolates were included in this study. All isolates were identified at the species level based on nucleotide sequences of the 16S ribosomal RNA gene and/or DNA-dependent RNA polymerase β-subunit gene (rpoB). Where necessary, isolates were identified based on whole genome sequences. Among them, 47 isolates were used for updating the ASTA database, and 182 isolates were used for the validation of Fusobacterium spp. identification.
Results: Fusobacterium isolates used for validation (182/182) were correctly identified at the genus level, and most (180/182) were correctly identified at the species level using the ASTA MicroIDSys system. Most of the F. nucleatum isolates (74/75) were correctly identified at the subspecies level.
Conclusion: The updated ASTA MicroIDSys system can identify nine species of Fusobacterium and four subspecies of F. nucleatum in good agreement. This tool can be routinely used in clinical microbiology laboratories to identify Fusobacterium species and serve as a springboard for future research.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Clinical Differences in Patients Infected with Fusobacterium and Antimicrobial Susceptibility of Fusobacterium Isolates Recovered at a Tertiary-Care Hospital in Korea
Annals of Laboratory Medicine
2022 .03
-
Fusobacterium nucleatum: caution with interpreting historical patient sample cohort
Journal of Pathology and Translational Medicine
2021 .11
-
새로운 말디토프 질량 분석 장비인 ASTA MicroIDSys의 임상 균주를 이용한 균종 동정 성능 평가
Annals of Clinical Microbiology
2020 .01
-
Fusobacterium Isolates Recovered From Colonic Biopsies of Inflammatory Bowel Disease Patients in Korea
Annals of Laboratory Medicine
2016 .01
-
Comparison of a New Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Platform, ASTA MicroIDSys, With Bruker Biotyper for Species Identification
Annals of Laboratory Medicine
2017 .01
-
Identification of Candida auris and Closely Related Species Using a New Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry, the ASTA MicroIDSys System
Laboratory Medicine Online
2022 .01
-
Association of Fusobacterium nucleatum in the Progression of Colorectal Cancer
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2021 .06
-
Performance of the ASTA MicroIDSys, a Novel MALDI-TOF MS Platform for Identifying Various Acinetobacter Species: Comparison with the Bruker Biotyper
Laboratory Medicine Online
2022 .07
-
Fusobacterium nucleatum infection induces CSF3 expression through the p38 MAPK and JNK signaling pathways in oral squamous cell carcinoma cells
International Journal of Oral Biology
2024 .03
-
Fusobacterium nucleatum stimulates dental pulp cells to produce prostaglandin E2 via mitogen-activated protein kinases activation
Oral Biology Research
2021 .12
-
Application of the Whole Genome-Based Bacterial Identification System, TrueBac ID, Using Clinical Isolates That Were Not Identified With Three Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Systems
Annals of Laboratory Medicine
2019 .01
-
Next generation sequencing analysis of exosomal microRNAs: Fusobacterium nucleatum regulates expression profiling of exosomal microRNAs in human colorectal cancer cells
International Journal of Oral Biology
2020 .01
-
Prognostic and clinicopathological significance of Fusobacterium nucleatum in colorectal cancer: a systemic review and meta-analysis
Journal of Pathology and Translational Medicine
2022 .05
-
The Link between Fusobacteria and Colon Cancer: a Fulminant Example and Review of the Evidence
Immune Network
2020 .08
-
Role of γ-glutamyltranspeptidase in osteoclastogenesis induced by Fusobacterium nucleatum
International Journal of Oral Biology
2021 .09
-
Development of a Novel Subunit Vaccine Targeting Fusobacterium nucleatum FomA Porin Based on In Silico Analysis
International Journal of Oral Biology
2017 .01
-
Prognostic Impact of Fusobacterium nucleatum Depends on Combined Tumor Location and Microsatellite Instability Status in Stage II/III Colorectal Cancers Treated with Adjuvant Chemotherapy
Journal of Pathology and Translational Medicine
2019 .01
-
The Interactive Effect of These Bacterial Substrates on the Growth of Streptococcus gordonii, Fusobacterium nucleatum and Porphyromonas gingivalis
치위생과학회지
2015 .01
-
An in vitro model of Fusobacterium nucleatum and Porphyromonas gingivalis in single- and dual-species biofilms
Journal of Periodontal & Implant Science
2018 .01
-
Substantial Improvement in Nontuberculous Mycobacterial Identification Using ASTA MicroIDSys Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry with an Upgraded Database
Annals of Laboratory Medicine
2022 .05
이 논문의 저자 정보
최근 본 자료
댓글(0)
0