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자료유형
학술저널
저자정보
Khadija Ahansal (Biotechnology Unit National Institute of Agronomi) Hanane Aadel (Biotechnology Unit National Institute of Agronomi) Sripada Mahabala Udupa (International Center for Agricultural Research in) Fatima Gaboun (Biotechnology Unit National Institute of Agronomi) Rabha Abdelwahd (Biotechnology Unit National Institute of Agronomi) Mohammed Ibriz (Ibn Tofail University) Driss Iraqi (Ibn Tofail University)
저널정보
한국식물생명공학회 Journal of Plant Biotechnology Journal of Plant Biotechnology 제49권 제2호
발행연도
2022.6
수록면
131 - 138 (8page)

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An efficient genetic transformation protocol is a fundamental requirement for high regeneration capacity from cultivated durum wheat (Triticum durum) varieties. In this study, wereportedtheeffectsoftwoauxins,2,4-dichloro phenoxyaceticacid(2,4-D)and4-amino-3,5,6-trichloropicoli nicacid(picloram), at a concentration of 2 mg/Laloneandin combination on the embryogenic callus and plantlet regeneration of four durum wheat varieties (Amria, Chaoui, Marouane, and Tomouh) using mature embryos (MEs) and immature embryos (ImEs). Significanteffectsofvariety,cul turemedium(theauxinused),andvariety-mediuminteraction were observed on the callus weight and plantlet regeneration of both MR and ImE explants. The medium used for callus induction significantly affected plantlet regeneration (p < 0.001). Comparedto2,4-D, picloram led to a higher plantlet regeneration rate in both ME and ImE explants (19.8% and 40.86%, respectively). Plantlet regeneration also varied significantly depending on the variety and medium used. PicloramledtohighplantletregenerationofbothME and ImE explants in all varieties except Tomouh, which showed high plantlet regeneration of ME explants in 2,4-D. A comparison of ME and ImE responses indicated that ImEs are the best explants for high plantlet regeneration in durum wheat. Ourfindingssuggestthatpicloramisthebestauxin and should be used instead of 2,4-D due to its positive effect on increasing plant regeneration of durum wheat ME and ImE explants.

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