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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.3
- 수록면
- 178 - 185 (8page)
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초록· 키워드
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Nitrogen is one of the most important mineral elements for plant growth. We studied the functional roles of Oryza sativa DNA BINDING WITH ONE FINGER 18 (OsDOF18) in controlling ammonium uptake. The growth of null mutants of OsDOF18 was retarded in a medium containing ammonium as the sole nitrogen source. In contrast, those mutants grew normally in a medium with nitrate as the sole nitrogen source. The gene expression was induced by ammonium but not by nitrate. Uptake of ammonium was lower in osdof18 mutants than in the wild type, while that of nitrate was not affected by the mutation. This indicated that OsDOF18 is involved in regulating ammonium transport. Among the 10 ammonium transporter genes examined here, expression of OsAMT1;1, OsAMT1;3, OsAMT2;1, and OsAMT4;1 was reduced in osdof18 mutants, demonstrating that the ammonium transporter genes function downstream of OsDOF18. Genes for nitrogen assimilation were also affected in the mutants. These results provide evidence that OsDOF18 mediates ammonium transport and nitrogen distribution, which then affects nitrogen use efficiency.
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참고문헌 (44)
참고문헌 신청
An, S. Y. / 2003 / Generation and analysis of end sequence database for T-DNA tagging lines in rice / Plant Physiol 133:2040~2047
An, G. / 2005 / Reverse genetic approaches for functional genomics of rice / Plant Mol. Biol 59:111~123
An, G. / 2005 / Molecular genetics using T-DNA in rice / Plant Cell Physiol 46:14~22
Bao, A. / 2015 / Overexpressing of OsAMT1-3, a high affinity ammonium transporter gene, modifies rice growth and carbon-nitrogen metabolic status / Int. J. Mol. Sci 16:9037~9063
Bloom, A. J. / 1992 / Root respiration associated with ammonium and nitrate absorption and assimilation by barley / Plant Physiol 99:1294~1301
An, G. / 2005 / Reverse genetic approaches for functional genomics of rice / Plant Mol. Biol 59:111~123
An, G. / 2005 / Molecular genetics using T-DNA in rice / Plant Cell Physiol 46:14~22
Bao, A. / 2015 / Overexpressing of OsAMT1-3, a high affinity ammonium transporter gene, modifies rice growth and carbon-nitrogen metabolic status / Int. J. Mol. Sci 16:9037~9063
Bloom, A. J. / 1992 / Root respiration associated with ammonium and nitrate absorption and assimilation by barley / Plant Physiol 99:1294~1301
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