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논문 기본 정보

자료유형
학술저널
저자정보
김혜정 (동아대학교) 조현석 (동아대학교) 박정헌 (동아대학교) 권택민 (동아대학교) 이재현 (동아대학교) 김도훈 (동아대학교) 이동희 (Genomine Inc.) 김창기 (한국생명공학연구원) 정영수 (동아대학교)
저널정보
한국분자세포생물학회 Molecules and Cells Molecules and Cells 제41권 제5호
발행연도
2018.3
수록면
413 - 422 (10page)
DOI
10.14348/molcells.2018.2254

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Soybean transgenic plants with ectopically expressed At-ABF3 were produced by Agrobacterium-mediated transformation and investigated the effects of AtABF3 expression on drought and salt tolerance. Stable Agrobacterium-mediated soybean transformation was carried based on the half-seed method (Paz et al. 2006). The integration of the transgene was confirmed from the genomic DNA of transformed soybean plants using PCR and the copy number of transgene was determined by Southern blotting using leaf samples from T2 seedlings. In addition to genomic integration, the expression of the transgenes was analyzed by RT-PCR and most of the transgenic lines expressed the transgenes introduced. The chosen two transgenic lines (line #2 and #9) for further experiment showed the substantial drought stress tolerance by surviving even at the end of the 20-day of drought treatment. And the positive relationship between the levels of AtABF3 gene expression and drought-tolerance was confirmed by qRT-PCR and drought tolerance test. The stronger drought tolerance of transgenic lines seemed to be resulted from physiological changes. Transgenic lines #2 and #9 showed ion leakage at a significantly lower level (P < 0.01) than non-transgenic (NT) control. In addition, the chlorophyll contents of the leaves of transgenic lines were signif-icantly higher (P < 0.01). The results indicated that their enhanced drought tolerance was due to the prevention of cell membrane damage and maintenance of chlorophyll content. Water loss by transpiration also slowly proceeded in transgenic plants. In micro scopic observation, higher stomata closure was confirmed in transgenic lines. Especially, line #9 had 56% of completely closed stomata whereas only 16% were completely open. In subsequent salt tolerance test, the apparently enhanced salt tolerance of transgenic lines was measured in ion leakage rate and chlorophyll contents. Finally, the agronomic characteristics of ectopically expressed AtABF3 transgenic plants (T2) compared to NT plants under regular watering (every 4 days) or low rate of watering condition (every 10 days) was investigated. When watered regularly, the plant height of drought-tolerant line (#9) was shorter than NT plants. However, under the drought condition, total seed weight of line #9 was significantly higher than in NT plants (P < 0.01). Moreover, the pods of NT plants showed severe withering, and most of the pods failed to set normal seeds. All the evidences in the study clearly suggested that overexpression of the AtABF3 gene conferred drought and salt tolerance in major crop soybean, especially under the growth condition of low watering.
#Agrobacterium-mediated transformation #AtABF3 #drought tolerance #soybean #stomatal closure

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참고문헌 (46)

참고문헌 신청
Abdeen, A. / 2010 / Transcriptome analysis reveals absence of unintended effects in drought-tolerant transgenic plants overexpressing the transcription factor ABF3 / BMC Genomics 11:69 Crossref Bruce, W.B. / 2002 / Molecular and physiological approaches to maize improvement for drought tolerance / J. Exp. Bot. 53:13~25 Crossref Choi, H.I. / 2000 / ABFs, a family of ABA-responsive element binding factors / J Biol Chem. 275:1723~1730 Crossref Yun-Sung Choi / 2013 / Overexpression of Arabidopsis ABF3 gene confers enhanced tolerance to drought and heat stress in creeping bentgrass / Plant Biotechnology Reports 7(2):165~173 dbpia Dan, Y. / 2008 / Biological functions of antioxidants in plant transformation / In Vitro Cell. Dev. Biol. Plant 44:149~161 Crossref

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