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논문 기본 정보

자료유형
학술저널
저자정보
Han Pei-pei (State Key Laboratory of Food Nutrition and Safety Key Laboratory of Industrial Fermentation Microbi) Geng Wen-ji (State Key Laboratory of Food Nutrition and Safety Key Laboratory of Industrial Fermentation Microbi) Li Meng-nan (State Key Laboratory of Food Nutrition and Safety Key Laboratory of Industrial Fermentation Microbi) Jia Shi-ru (State Key Laboratory of Food Nutrition and Safety Key Laboratory of Industrial Fermentation Microbi) Yin Ji-long (Tianjin Research Institute for Water Transportation Engineering M.O.T. Tianjin 300456 P.R. China) Xue Run-ze (Tianjin Research Institute for Water Transportation Engineering M.O.T. Tianjin 300456 P.R. China)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제31권 제9호
발행연도
2021.9
수록면
1,311 - 1,322 (12page)
DOI
10.4014/jmb.2104.04019

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Microbially induced calcium carbonate precipitation (MICP) has recently become an intelligent and environmentally friendly method for repairing cracks in concrete. To improve on this ability of microbial materials concrete repair, we applied random mutagenesis and optimization of mineralization conditions to improve the quantity and crystal form of microbially precipitated calcium carbonate. Sporosarcina pasteurii ATCC 11859 was used as the starting strain to obtain the mutant with high urease activity by atmospheric and room temperature plasma (ARTP) mutagenesis. Next, we investigated the optimal biomineralization conditions and precipitation crystal form using Plackett-Burman experimental design and response surface methodology (RSM). Biomineralization with 0.73 mol/l calcium chloride, 45 g/l urea, reaction temperature of 45°C, and reaction time of 22 h, significantly increased the amount of precipitated calcium carbonate, which was deposited in the form of calcite crystals. Finally, the repair of concrete using the optimized biomineralization process was evaluated. A comparison of water absorption and adhesion of concrete specimens before and after repairs showed that concrete cracks and surface defects could be efficiently repaired. This study provides a new method to engineer biocementing material for concrete repair.

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