지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.12
- 수록면
- 290 - 295 (6page)
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초록· 키워드
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Objectives: Dental plaque emits red fluorescence under a visible blue light near the ultra-violet end of the light spectrum. The fluorescence characteristics of each microorganism have been reported in several studies. The aim of this study was to evaluate changes in red fluorescence of oral microorganisms that is affected by blood in the culture media.
Methods: The gram-positive Actinomyces naeslundii (AN, KCTC 5525) and Lactobacillus casei (LC, KCTC 3109) and gram negative Prevotella intermedia (PI, KCTC 3692) that are known to emit red fluorescence were used in this study. Each bacterium was activated in broth and cultivated in different agar media at 37oC for 7 days. Tryptic soy agar with hemin and vitamin K3 (TSA), TSA with sheep blood (TSAB), basal medium mucin (BMM) medium, and BMM with sheep blood (BMMB) were used in this study. Fluorescence due to bacterial growth was observed under 405-nm wavelength blue light using the quantitative light-induced fluorescence-digital (QLF-D) device. The red, green, and blue fluorescence values of colonies were obtained using image-analysis software and the red to green ratio (R/G value) and red to total RGB ratio (R/RGB value) were calculated for quantitative comparison.
Results: The QLF-D images of the AN, LC, and PI colonies showed red fluorescence in all media, but the fluorescence of all bacteria was reduced in TSA and BMM media, compared with in TSAB and BMMB media. Both the R/G and the R/RGB values of all bacteria were significantly reduced in growth media without blood (P<0.001).
Conclusions: Based on this in vitro study, it can be concluded that red fluorescence of oral bacteria can be affected by growth components, especially blood. Blood-containing medium could be a significant factor influencing red fluorescence of oral bacteria. It can be further hypothesized that bleeding in the oral cavity can increase the red fluorescence of dental plaque.
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등록된 정보가 없습니다.
참고문헌 (18)
참고문헌 신청
Kolenbrander PE / 2010 / Oral multispecies biofilm development and the key role of cell - cell distance / Nat Rev Microbiol 8:471~480
Zijnge V / 2010 / Oral biofilm architecture on natural teeth / PloS one 24(5(2)):e9321
van der Veen MH / 2000 / Application of quantitative light-induced fluorescence for assessing early caries lesions / Monogr Oral Sci 17:144~162
Coulthwaite L / 2006 / The microbiological origin of fluorescence observed in plaque on dentures during QLF analysis / Caries Res 40:112~116
Koenig K / 1994 / In-vivo fluorescence detection and imaging of porphyrinproducing bacteria in the human skin and in the oral cavity for diagnosis of acne vulgaris, caries, and squamous cell carcinoma / International Society for Optics and Photonics 2135:129~139
Zijnge V / 2010 / Oral biofilm architecture on natural teeth / PloS one 24(5(2)):e9321
van der Veen MH / 2000 / Application of quantitative light-induced fluorescence for assessing early caries lesions / Monogr Oral Sci 17:144~162
Coulthwaite L / 2006 / The microbiological origin of fluorescence observed in plaque on dentures during QLF analysis / Caries Res 40:112~116
Koenig K / 1994 / In-vivo fluorescence detection and imaging of porphyrinproducing bacteria in the human skin and in the oral cavity for diagnosis of acne vulgaris, caries, and squamous cell carcinoma / International Society for Optics and Photonics 2135:129~139
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