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논문 기본 정보

자료유형
학술저널
저자정보
(전북대학교) (전북대학교) (전북대학교) (전북대학교) (전북대학교) (전북대학교) (University of Minho Portugal) (University of Minho Portugal) (전북대학교)
저널정보
한국생체재료학회 생체재료학회지 생체재료학회지 제21권 제1호
발행연도
수록면
10 - 16 (7page)
DOI
10.1186/s40824-017-0088-4

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초록· 키워드

Background: The aim of this study was to investigate the osteogenesis effects of DC and DC/HAp sponge immersed in without and with dexamethasone. Methods: The experimental groups in this study were DC and DC/HAp sponge immersed in without dexamethasone (Dex(?)DC and Dex(?)-DC/HAp group) and with dexamethasone (Dex(+)-DC and Dex(+)-DC/HAp group). We characterized DC and DC/HAp sponge using compressive strength, scanning electron microscopy (SEM). Also, osteogenic differentiation of BMSCs on sponge (Dex(?)DC, Dex(?)-DC/HAp, Dex(+)-DC and Dex(+)-DC/HAp group) was assessed by SEM, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (MTT) assay, alkaline phosphatase (ALP) activity assay and reverse transcription-PCR (RT-PCR). Results: In this study, we assessed osteogenic differentiation of BMSCs on Duck’s feet-derived collagen (DC)/HAp sponge immersed with dexamethasone Dex(+)-DC/HAp. These results showed that Dex(+)-DC/HAp group increased cell proliferation and osteogenic differentiation of BMSCs during 28 days. Conclusion: From these results, Dex(+)-DC/HAp can be envisioned as a potential biomaterial for bone regeneration applications.
#Duck’s feet-derived collagen #Hydroxyapatite #Sponges #Dexamethasone #Osteogenesis #Bone regeneration
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