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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.12
- 수록면
- 136 - 140 (5page)
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Lam6 is a member of sterol-specific lipid transfer proteins anchored at membrane contact sites (LAMs). Lam6 localizes tothe ER-mitochondria contact sites by its PH-like domain and the C-terminal transmembrane helix. Here, we purified andcrystallized the Lam6 PH-like domain from Saccharomyces cerevisiae. To aid crystallization of the Lam6 PH-like domain, T4lysozyme was fused to the N-terminus of the Lam6 PH-like domain with a short dipeptide linker, GlySer. The fusion proteinwas crystallized under the condition of 0.1 M HEPES-HCl pH 7.0, 10% (w/v) PEG 8000, and 0.1 M Na3Citrate at 293K. X-raydiffraction data of the crystals were collected to 2.4 A resolution using synchrotron radiation. The crystals belong to theorthorhombic space group P212121 with unit cell parameters a = 59.5 A, b = 60.1 A, and c = 105.6 A. The asymmetric unitcontains one T4L-Lam6 molecule with a solvent content of 58.7%. The initial attempt to solve the structure by molecularreplacement using the T4 lysozyme structure was successful.
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참고문헌 (17)
참고문헌 신청
Elbaz-Alon, Y / 2015 / Lam6 Regulates the Extent of Contacts between Organelles / Cell Rep 12:7~14
Emsley, P / 2010 / Features and development of Coot / Acta Crystallogr D Biol Crystallogr 66:486~501
Gatta, A. T / 2015 / A new family of StART domain proteins at membrane contact sites has a role in ER-PM sterol transport / Elife 4
Helle, S. C / 2013 / Organization and function of membrane contact sites / Biochim Biophys Acta 1833:2526~2541
Holthuis, J. C / 2005 / Lipid traffic : floppy drives and a superhighway / Nat Rev Mol Cell Biol 6:209~220
Emsley, P / 2010 / Features and development of Coot / Acta Crystallogr D Biol Crystallogr 66:486~501
Gatta, A. T / 2015 / A new family of StART domain proteins at membrane contact sites has a role in ER-PM sterol transport / Elife 4
Helle, S. C / 2013 / Organization and function of membrane contact sites / Biochim Biophys Acta 1833:2526~2541
Holthuis, J. C / 2005 / Lipid traffic : floppy drives and a superhighway / Nat Rev Mol Cell Biol 6:209~220
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