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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한임상검사정도관리협회 Journal of Laboratory Medicine And Quality Assurance Laboratory Medicine and Quality Assurance 제43권 제3호
- 발행연도
- 2021.9
- 수록면
- 152 - 161 (10page)
- DOI
- 10.15263/jlmqa.2021.43.3.152
이용수
초록· 키워드
오류제보하기
Background: Flow cytometry crossmatch (FCXM) is the most sensitive method currently used for human leukocyte antigen (HLA) crossmatches. The FCXM test methods and cut-off values used to determine the positive reaction vary significantly in different laboratories. To obtain comparable FCXM results from different laboratories and to use these results in deciding the compatibility between a donor and a recipient in organ transplantation, a standardized protocol needs to be established. In this study, we attempted to develop a harmonized test method and verify its performance with that of the different laboratories’ methods through a multicenter comparison.
Methods: The harmonized method was determined via a literature review and a survey that included seven laboratories. For the harmonized method, cell number, serum amount, pronase treatment for B cell FCXM, incubation temperature and time, and anti-human immunoglobulin G fluorescein isothiocyanate conjugate were standardized. Two trials of FCXM using one cell and three sera samples in each trial were performed, and the results of the laboratories’ methods versus those of the harmonized method were analyzed.
Results: Compared to the laboratory methods, the harmonized method showed increased agreement with consensus positive/negative results (75/84, 89.3% vs. 81/84, 96.4%) and a significantly decreased coefficient of variation among different laboratories in detecting the median fluorescence intensity (MFI) ratio values (88.3% vs. 55.2%, P =0.0003).
Conclusions: When the same protocol for the FCXM method is used, an increased consensus of positive/negative results and decreased variation of the MFI ratios from different laboratories can be obtained. Implementation of a harmonized and standardized protocol is needed in all domestic laboratories that perform the FCXM tests.
목차
등록된 정보가 없습니다.
참고문헌 (16)
참고문헌 신청
Garovoy MR / 1983 / Flow cytometry analysis: a high technology cross-match technique facilitating transplantation / Transplant Proc 15:1939~1944
Graff RJ / 2010 / The clinical importance of flow cytometry crossmatch in the context of CDC crossmatch results / Transplant Proc 42:3471~3474
Tinckam K / 2009 / Histocompatibility methods / Transplant Rev (Orlando) 23:80~93
Ayna TK / 2013 / Comparison of complement-dependent cytotoxic and flow-cytometry crossmatch results before cadaveric kidney transplantation / Transplant Proc 45:878~880
Eun-Suk Kang / 2019 / Annual Report of Korean Association of External Quality Assessment Service on Histocompatibility Testing (2018) / Laboratory Medicine and Quality Assurance 41(3):130~152
Graff RJ / 2010 / The clinical importance of flow cytometry crossmatch in the context of CDC crossmatch results / Transplant Proc 42:3471~3474
Tinckam K / 2009 / Histocompatibility methods / Transplant Rev (Orlando) 23:80~93
Ayna TK / 2013 / Comparison of complement-dependent cytotoxic and flow-cytometry crossmatch results before cadaveric kidney transplantation / Transplant Proc 45:878~880
Eun-Suk Kang / 2019 / Annual Report of Korean Association of External Quality Assessment Service on Histocompatibility Testing (2018) / Laboratory Medicine and Quality Assurance 41(3):130~152
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