AC092127.1-miR-451a-AE binding protein 2 Signaling Facilitates Malignant Properties of Breast Cancer

Xiumei Zhang; Lin Cong; Dafang Xu; Qi Leng; Ming Shi; Yonghua Zhou

doi:10.4048/jbc.2021.24.e37

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자료유형: 학술저널

저자정보: Xiumei Zhang (Department of Pathology the People's Hospital of Xinghua City Xinghua China.) Lin Cong (Department of Pathology the People's Hospital of Xinghua City Xinghua China.) Dafang Xu (Department of Pathology the People's Hospital of Xinghua City Xinghua China.) Qi Leng (Department of Pathology the People's Hospital of Xinghua City Xinghua China.) Ming Shi (Department of Pathology the People's Hospital of Xinghua City Xinghua China.) Yonghua Zhou (Department of Pathology the People's Hospital of Xinghua City Xinghua China.)

저널정보: 한국유방암학회 Journal of Breast Cancer Journal of Breast Cancer Vol.24 No.4

발행연도: 2021.8

수록면: 389 - 401 (13page)

DOI: 10.4048/jbc.2021.24.e37

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Purpose: The purpose of the current study was to explore the functions and potential mechanism of miR-451a in breast cancer (BC). Methods: Quantitative reverse transcription real-time polymerase chain reaction was used to analyze the expression of miR-451a in human normal mammary cells (MCF-10A) and BC cells. Colony formation assay, terminal-deoxynucleoitidyl transferase mediated nick end labeling assay and transwell assays were conducted to validate the effect of miR-451a on proliferation, apoptosis, migration and invasion of BC cells, respectively. RNA pull-down, RNA immunoprecipitation and luciferase reporter assays were applied to investigate the upstream and downstream mechanisms of miR-451a in BC cells. Results: MiR-451a was expressed at a low level in BC cells. Overexpression of miR-451a repressed BC cells proliferation, migration and invasion. Moreover, long non-coding RNA AC092127.1 acted as a sponge of miR-451a to enhance the expression level of AE binding protein 2 (AEBP2) that was demonstrated to be the target gene of miR-451a in BC cells. Finally, rescue experiments validated that miR-451a and AEBP2 involved in AC092127.1-mediated BC cell growth, migration and invasion. Conclusion: In a word, AC092127.1/miR-451a/AEBP2 axis contributes to BC cell growth, migration and invasion. Our results may help to find novel potential targets for BC treatment.

#Breast neoplasms #Cell proliferation #MicroRNAs #RNA #long noncoding

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참고문헌 신청

McDonald ES / 2016 / Clinical diagnosis and management of breast cancer / J Nucl Med 57(Suppl 1):9S~16S

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