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논문 기본 정보

자료유형
학술저널
저자정보
Sathish K. Ponniah (University of Arkansas at Pine Bluff) Zhenhua Shang (University of Arkansas at Pine Bluff) M. Aydın Akbudak (University of Arkansas) Vibha Srivastava (University of Arkansas) Muthusamy Manoharan (University of Arkansas at Pine Bluff)
저널정보
한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제11권 제1호
발행연도
2017.2
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17 - 27 (11page)

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Rice straw is one of the largest biomasses in the world that can potentially be exploited for biofuel. Nevertheless, the association of lignin with cellulose and hemicellulose has hindered the efficient utilization of rice straw for cellulosic biofuel. The objective of this study was to down-regulate key genes involved in lignin biosynthesis pathway, such as hydroxycinnamoyl CoA: shikimate hydroxycinnamoyl transferase (HCT), cinnamoyl CoA reductase (CCR), and cinnamyl alcohol dehydrogenase (CAD), through “terminator-less” constructs to reduce lignin in transgenic rice. Real-time qPCR analyses of the selected T1 transgenic rice plants indicated 36?86% transcript reduction in HCT lines, 75?94% in CCR lines, and 10?85% in CAD lines. Of the nine down-regulated lines (three lines from each genes) subjected to lignin analysis, seven showed significant reduction in total lignin content (HCT-4, HCT-7, CAD-1, CAD-7, CCR-3, CCR-7, and CCR-12) with lignin reduction ranging from 4.6 to 10.8%. The results from this study indicated that truncated gene fragments lacking transcription termination sequence can be used for down-regulation of lignin genes in rice, and the rice straw from these transgenic lines could be useful as feedstock for cellulosic biofuel.

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