지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.2
- 수록면
- 39 - 46 (8page)
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The FLOWERING TIME CONTROL PROTEIN FPA (FPA) gene encodes an RNA recognition motif (RRM) domain protein and plays an important role in flowering time control. Flowering responds to environmental conditions and developmental regulation through a network of signaling pathways. However, a little is known about the functions of autonomous pathway genes in Medicago truncatula. Here, we characterized the M. truncatula FPA (MtFPA) gene expression profiling through quantitative RT-PCR analysis, cellular localization, and functional analyses in transgenic plants. We cloned the FPA gene of M. truncatula based on its sequence similarity with Arabidopsis thaliana FPA. The quantitative RT-PCR analysis of MtFPA expression patterns showed that the MtFPA transcripts accumulated ubiquitously in the roots, leaves, stems, flowers, and pods of M. truncatula. The confocal image analysis of the fusion protein MtFPA:GFP revealed that MtFPA was localized in the nucleus. To examine the function of MtFPA, the 35S::MtFPA transgenic plants were generated in the Arabidopsis late-flowering mutant background, fpa-2. The overexpression of MtFPA accelerated flowering under long day conditions compared to the non-transgenic plants. In MtFPA transgenic lines, the expression of AtFLC was down-regulated, whereas that of the floral integrators, AtFT and AtSOC1, was up-regulated as compared to the control plants. These results suggest that MtFPA is a functional orthologue of Arabidopsis and plays an important role in the regulation of flowering time in legumes, especially in M. truncatula.
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참고문헌 (23)
참고문헌 신청
Barker DG / 2006 / The Medicago truncatula handbook / Samuel Roberts Noble Foundation
Baurle I / 2007 / Widespread role for the flowering-time regulators FCA and FPA in RNA-mediated chromatin silencing / Science 318:109~112
Chen SY / 2007 / OsRRM, a Spen-like rice gene expressed specifically in the endosperm / Cell Res 17:713~721
Clough SJ / 1998 / Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana / Plant J 16:735~743
Fernandez V / 2016 / Photoperiodic and thermosensory pathways interact through CONSTANS to promote flowering at high temperature under short days / Plant J 86:426~440
Baurle I / 2007 / Widespread role for the flowering-time regulators FCA and FPA in RNA-mediated chromatin silencing / Science 318:109~112
Chen SY / 2007 / OsRRM, a Spen-like rice gene expressed specifically in the endosperm / Cell Res 17:713~721
Clough SJ / 1998 / Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana / Plant J 16:735~743
Fernandez V / 2016 / Photoperiodic and thermosensory pathways interact through CONSTANS to promote flowering at high temperature under short days / Plant J 86:426~440
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