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자료유형
학술저널
저자정보
최정우 (University of Guelph) 이성진 (강원대학교) Xiaoping Liao (University of Alberta) 박새롬 (강원대학교) 전현정 (농업진흥청) 정원홍 (한국생명공학연구원) Paul Stothard (University of Alberta) Yeon Soo Park (Gangwon Provincial Livestock Research Center Heongseong Korea) 이정구 (강원대학교) 이경태 (농촌진흥청) 김상환 (Hankyong National University) 오재돈 (한경대학교) 김남신 (한국생명공학연구원) Tae-Hun Kim (Rural Development Administration) Hak Kyo Lee (Hankyong National University)
저널정보
한국분자세포생물학회 Molecules and Cells Molecules and Cells 제36권 제3호
발행연도
2013.9
수록면
203 - 211 (9page)
DOI
10.1007/s10059-013-2347-0

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Since the completion of the bovine sequencing projects, a substantial number of genetic variations such as single nucleotide polymorphisms have become available across the cattle genome. Recently, cataloguing such genetic variations has been accelerated using massively parallel sequencing technology. However, most of the recent studies have been concentrated on European Bos taurus cattle breeds, resulting in a severe lack of knowledge for valuable native cattle genetic resources worldwide. Here, we present the first whole-genome sequencing results for an endangered Korean native cattle breed, Chikso, using the Illumina HiSeq 2,000 sequencing platform. The genome of a Chikso bull was sequenced to approximately 25.3-fold coverage with 98.8% of the bovine reference genome sequence (UMD 3.1) covered. In total, 5,874,026 single nucleotide polymorphisms and 551,363 insertion/deletions were identified across all 29 autosomes and the X-chromosome, of which 45% and 75% were previously unknown, respectively. Most of the variations (92.7% of single nucleotide polymorphisms and 92.9% of insertion/deletions) were located in intergenic and intron regions. A total of 16,273 single nucleotide polymorphisms causing missense mutations were detected in 7,111 genes throughout the genome, which could potentially contribute to variation in economically important traits in Chikso. This study provides a valuable resource for further investigations of the genetic mechanisms underlying traits of interest in cattle, and for the development of improved genomics-based breeding tools.

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