지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.7
- 수록면
- 530 - 535 (6page)
- DOI
- 10.14348/molcells.2016.0033
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초록· 키워드
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Large conductance calcium-activated potassium (BK) channels participate in many important physiological functions in excitable tissues such as neurons, cardiac and smooth muscles, whereas the knowledge of BK channels in bone tissues and osteoblasts remains elusive. To investigate the role of BK channels in osteoblasts, we used transcription activator-like effector nuclease (TALEN) to establish a BK knockout cell line on rat ROS17/2.8 osteoblast, and detected the proliferation and mineralization of the BK-knockout cells. Our study found that the BK-knockout cells significantly decreased the ability of proliferation and mineralization as osteoblasts, compared to the wild type cells. The overall expression of osteoblast differentiation marker genes in the BK-knockout cells was significantly lower than that in wild type osteoblast cells. The BK-knockout osteoblast cell line in our study displays a phenotype decrease in osteoblast function which can mimic the pathological state of osteoblast and thus provide a working cell line as a tool for study of osteoblast function and bone related diseases.
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참고문헌 (28)
참고문헌 신청
Bentzen, B. H. / 2014 / BK channel activators and their therapeutic perspectives / Front. Physiol 5:389
Black, J. A. / 2013 / Noncanonical roles of voltage-gated sodium channels / Neuron 80:280~291
Bruderer, M. / 2014 / Role and regulation of RUNX2 in osteogenesis / Eur. Cells Mater 28:269~286
Chen, J. / 2014 / An efficient antiviral strategy for targeting hepatitis B virus genome using transcription activator-like effector nucleases / Mol. Ther 22:303~311
Chen, Q. / 2015 / Up-regulatory effects of curcumin on large conductance Ca2+-activated K+ channels / PLoS One 10:0144800
Black, J. A. / 2013 / Noncanonical roles of voltage-gated sodium channels / Neuron 80:280~291
Bruderer, M. / 2014 / Role and regulation of RUNX2 in osteogenesis / Eur. Cells Mater 28:269~286
Chen, J. / 2014 / An efficient antiviral strategy for targeting hepatitis B virus genome using transcription activator-like effector nucleases / Mol. Ther 22:303~311
Chen, Q. / 2015 / Up-regulatory effects of curcumin on large conductance Ca2+-activated K+ channels / PLoS One 10:0144800
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