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논문 기본 정보

자료유형
학술저널
저자정보
Sanjay K. S. Patel (Konkuk University) Jae-Hoon Jeong (Konkuk University) Sanjeet Mehariya (Konkuk University) Sachin V. Otari (Konkuk University) Bharat Madan (Konkuk University) Jung Rim Haw (건국대학교) Jung-Kul Lee (Konkuk University) Liaoyuan Zhang (건국대학교) In-Won Kim (Konkuk University)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제12호
발행연도
2016.12
수록면
2,098 - 2,105 (8page)
DOI
10.4014/jmb.1608.08053

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Massive reserves of methane (CH4) remain unexplored as a feedstock for the production of liquid fuels and chemicals, mainly because of the lack of economically suitable and sustainable strategies for selective oxidation of CH4 to methanol. The present study demonstrates the bioconversion of CH4 to methanol mediated by Type I methanotrophs, such as Methylomicrobium album and Methylomicrobium alcaliphilum. Furthermore, immobilization of a Type II methanotroph, Methylosinus sporium, was carried out using different encapsulation methods, employing sodium-alginate (Na-alginate) and silica gel. The encapsulated cells demonstrated higher stability for methanol production. The optimal pH, temperature, and agitation rate were determined to be pH 7.0, 30oC, and 175 rpm, respectively, using inoculum (1.5 mg of dry cell mass/ml) and 20% of CH4 as a feed. Under these conditions, maximum methanol production (3.43 and 3.73 mM) by the encapsulated cells was recorded. Even after six cycles of reuse, the Na-alginate and silica gel encapsulated cells retained 61.8% and 51.6% of their initial efficiency for methanol production, respectively, in comparison with the efficiency of 11.5% observed in the case of free cells. These results suggest that encapsulation of methanotrophs is a promising approach to improve the stability of methanol production.

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