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논문 기본 정보

자료유형
학술저널
저자정보
Mi-Jeong Ahn (Gyeongnam National University of Science and Technology) 노설아 (고려대학교) 하선화 (농촌진흥청) 백경환 (전남대학교) 이신우 (경남과학기술대학교) 배정명 (고려대학교)
저널정보
한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제6권 제2호
발행연도
2012.4
수록면
133 - 140 (8page)

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One of the drawbacks in inducing the synthesis of ketocarotenoids in transgenic plants is the accompanying decrease in b-carotene level, especially in transgenic plants overexpressing only a b-carotene ketolase. In an attempt to overcome this drawback, we isolated a b-carotene ketolase (HpBkt) cDNA from Haematococcus pluvialis and generated transgenic carrot plants overexpressing HpBkt cDNA under the control of the ibAGP1 promoter and its transit peptide sequence. Semi-quantitative reverse transcriptase-PCR with total RNAs extracted from taproot tissues of five lines of the generated HpBkt-transgenic carrot plants revealed variable levels of the inserted HpBkt transcript, ranging from very low to high. All of the HpBkttransgenic carrot plants tested produced astaxanthin, resulting in a color change from the light-orange coloration of wild-type taproots to a dark-orange?reddish coloration in the taproots of the HpBkt-transgenic plants. The astaxanthin content was not correlated with the HpBkt transcript level. Overexpression of HpBkt caused an increase in the transcript levels of the endogenous carotenogenic genes, including phytoene synthase 1 (PSY1), phytoene synthase 2 (PSY2), lycopene b-cyclase 1 (LCYB1), and b-carotene hydroxylase 1 (CHXB1), which resulted in elevated b-carotene levels in the HpBkt-transgenic plants (range 1.3- to 2.5-fold) compared to the wild-type plants. These results demonstrate that HpBkt-overexpressing carrot plants under the control of the ibAGP1 promoter and its transit peptide are capable of both newly synthesizing ketocarotenoids and enhancing their b-carotene level.

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