지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.10
- 수록면
- 414 - 422 (9page)
이용수
초록· 키워드
오류제보하기
Eucalyptus dieback disease, caused by Cylindrocladium scoparium, has occurred in last few years in large Eucalyptus planting areas in China and other countries. Rapid, simple, and reliable diagnostic techniques are desired for the early detection of Eucalyptus dieback of C. scoparium prior to formulation of efficient control plan. For this purpose, three PCR-based methods of nested PCR, multiplex PCR, loop-mediated isothermal amplification (LAMP) were developed for detection of C. scoparium based on factor 1-alpha (tef1) and beta-tubulin gene in this study. All of the three methods showed highly specific to C. scoparium. The sensitivities of the nested PCR and LAMP were much higher than the multiplex PCR. The sensitivity of multiplex PCR was also higher than regular PCR. C. scoparium could be detected within 60 min from infected Eucalyptus plants by LAMP, while at least 2 h was needed by the rest two methods. Using different Eucalyptus tissues as samples for C. scoparium detection, all of the three PCR-based methods showed much better detection results than regular PCR. Base on the results from this study, we concluded that any of the three PCR-based methods could be used as diagnostic technology for the development of efficient strategies of Eucalyptus dieback disease control. Particularly, LAMP was the most practical method in field application because of its one-step and rapid reaction, simple operation, single-tube utilization, and simple visualization of amplification products.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Application Study of PCR Additives to Improve The Split Peaks in Direct PCR
한국분석과학회 학술대회
2019 .05
-
Nested PCR을 이용하여 조직으로부터 Mycobacterium tuberculosis Complex 신속검출
대한임상검사과학회지
2015 .01
-
Development of a Multiplex PCR for Rapid and Simultaneous Detection of Staphylococcus Species
한국미생물학회 학술대회논문집
2022 .10
-
Highly Sensitive Detection of Low-Abundance White Spot Syndrome Virus by a Pre-Amplification PCR Method
Journal of Microbiology and Biotechnology
2017 .01
-
Occurrence and Multiplex PCR Detection of Citrus Yellow Vein Clearing Virus in Korea
The Plant Pathology Journal
2024 .04
-
Simultaneous Detection of Four Foodborne Viruses in Food Samples Using a One-Step Multiplex Reverse Transcription PCR
Journal of Microbiology and Biotechnology
2018 .01
-
화상병원세균 검출을 위한 Conventional PCR 향상
Research in Plant Disease(식물병연구)
2024 .09
-
Optimization and Application of Multiplex Nested PCR to Detect Free-living Amoeba in Biomass-rich Samples
한국미생물학회 학술대회논문집
2021 .08
-
Application study of PCR additives to improve the split peaks in direct PCR
분석과학
2019 .08
-
박과 작물에 과일썩음병을 일으키는 Acidovorax citrulli 검출을 위한 nested-PCR 검사법 개발
Research in Plant Disease(식물병연구)
2015 .06
-
Diagnosis of Viral Infection Using Real-time Polymerase Chain Reaction
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2018 .03
-
대기입자상물질시료의곰팡이메타게놈분석을위한 DNA추출및 PCR조건최적화
한국미생물·생명공학회지
2023 .03
-
Development of PCR and TaqMan PCR Assays to Detect Pseudomonas coronafaciens, a Causal Agent of Halo Blight of Oats
The Plant Pathology Journal
2015 .03
-
Functional genomics by integrated analysis of transcriptome of sweet potato (Ipomoea batatas (L.) Lam.) during root formation
Genes & Genomics
2020 .01
-
Evaluation of Multiplex Polymerase Chain Reaction Assay for the Simultaneous Detection of Sexually Transmitted Infections Using Swab Specimen
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2020 .03
-
Optimization and Application of Multiplex PCR Method to Detect Genes Involved in Cyanotoxin Synthesis
한국미생물학회 학술대회논문집
2021 .08
-
A Multiplex-PCR for Rapid Identification of 4 Enterococcus Species Using Species-Specific Primers from Comparative Genomics
한국미생물학회 학술대회논문집
2016 .04
-
Ultra-fast digital PCR for COVID-19 detection
한국진공학회 학술발표회초록집
2020 .08
-
Genetic Diversity and Population Structure of Phyllosphere-Associated Xanthomonas euvesicatoria Bacteria in Physalis pubescens Based on BOX-PCR and ERIC-PCR in China
The Plant Pathology Journal
2025 .02
-
Loop-mediated Isothermal Amplification assay for Detection of Candidatus Liberibacter Asiaticus, a Causal Agent of Citrus Huanglongbing
The Plant Pathology Journal
2018 .12
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0102-2023-481-001834907